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Analytical and Bioanalytical Chemistry

, Volume 411, Issue 25, pp 6677–6686 | Cite as

Application of Cu2+-based electron spin resonance spectroscopy in measurement of antioxidant capacity of fruits

  • Sizhu Tian
  • Xuwen Li
  • Jia Jiang
  • Kun Wang
  • Hanqi Zhang
  • Aimin Yu
  • Ziwei ZhangEmail author
Research Paper

Abstract

The antioxidant capacity of 22 kinds of fruits was measured by the developed electron spin resonance (ESR) method based on Cu2+ sensor. Cu2+ is reduced to Cu+ by the antioxidants in the fruits, and the remaining Cu2+ was determined by ESR and UV-Vis spectroscopy. Cu2+ can give an ESR signal whereas Cu+ cannot, and the loss of the ESR signal was used to quantify the antioxidant capacity of various fruits. The results were shown as vitamin C equivalent antioxidant capacity (VCEAC). The VCEAC values obtained by ESR and UV-Vis methods ranged from 24.23 to 688.61 mg/100 g and from 24.12 to 677.79 mg/100 g, respectively. Cupric ion reducing antioxidant capacity (CUPRAC) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods were employed for comparison. Based on Pearson’s correlation test, the results obtained by CUPRAC and DPPH methods were both significantly correlated with these obtained by the present method, which indicated that the novel method was reliable. Total phenolic content for all kinds of fruits was measured with the Folin–Ciocalteu reagent, and VCEAC values obtained by the ESR method were significantly correlated with total phenolic contents.

Graphical abstract

Keywords

Electron spin resonance Cu2+ reduction Fruits Antioxidant capacity VCEAC Total phenolic content 

Notes

Funding information

This work was supported by the Science and Technology Development Planning Project of Jilin Province (Grant No. 20180201027GX and 20180201059SF) and the National Natural Science Foundation of China (Grant No. 31300621).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001;54:176–86.CrossRefGoogle Scholar
  2. 2.
    Droge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002;82:47–95.CrossRefGoogle Scholar
  3. 3.
    Jiang LY, He S, Pan YJ, Sun CR. Bioassay-guided isolation and EPR-assisted antioxidant evaluation of two valuable compounds from mango peels. Food Chem. 2010;119:1285–92.CrossRefGoogle Scholar
  4. 4.
    Stocker P, Lesgards JF, Vidal N, Chalier F, Prost M. ESR study of a biological assay on whole blood: antioxidant efficiency of various vitamins. Biochim Biophys Acta Gen Subj. 2003;1621:1–8.CrossRefGoogle Scholar
  5. 5.
    Poprac P, Jomova K, Simunkova M, Kollar V, Rhodes CJ, Valko M. Targeting free radicals in oxidative stress-related human diseases. Trends Pharmacol Sci. 2017;38:592–607.CrossRefGoogle Scholar
  6. 6.
    Chan C-L, Gan R-Y, Corke H. The phenolic composition and antioxidant capacity of soluble and bound extracts in selected dietary spices and medicinal herbs. Int J Food Sci Tech. 2016;51:565–73.CrossRefGoogle Scholar
  7. 7.
    Živković J, Zeković Z, Mujić I, Gođevac D, Mojović M, Mujić A, et al. EPR spin-trapping and spin-probing spectroscopy in assessing antioxidant properties: example on extracts of catkin, leaves, and spiny burs of castanea sativa. Food Biophys. 2009;4:126–33.CrossRefGoogle Scholar
  8. 8.
    Li Y, Bao T, Chen W. Comparison of the protective effect of black and white mulberry against ethyl carbamate-induced cytotoxicity and oxidative damage. Food Chem. 2018;243:65–73.CrossRefGoogle Scholar
  9. 9.
    Contreras-Calderón J, Calderón-Jaimes L, Guerra-Hernández E, García-Villanova B. Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Res Int. 2011;44:2047–53.CrossRefGoogle Scholar
  10. 10.
    Jiao Y, Kilmartin PA, Fan M, Quek SY. Assessment of phenolic contributors to antioxidant activity of new kiwifruit cultivars using cyclic voltammetry combined with HPLC. Food Chem. 2018;268:77–85.CrossRefGoogle Scholar
  11. 11.
    Jez M, Wiczkowski W, Zielinska D, Bialobrzewski I, Blaszczak W. The impact of high pressure processing on the phenolic profile, hydrophilic antioxidant and reducing capacity of puree obtained from commercial tomato varieties. Food Chem. 2018;261:201–9.CrossRefGoogle Scholar
  12. 12.
    Diaconeasa Z, Leopold L, Rugina D, Ayvaz H, Socaciu C. Antiproliferative and antioxidant properties of anthocyanin rich extracts from blueberry and blackcurrant juice. Int J Food Sci Tech. 2015;16:2352–65.Google Scholar
  13. 13.
    Wang H, Guo X, Hu X, Li T, Fu X, Liu RH. Comparison of phytochemical profiles, antioxidant and cellular antioxidant activities of different varieties of blueberry (Vaccinium spp.). Food Chem. 2017;217:773–81.CrossRefGoogle Scholar
  14. 14.
    Shahidi F, Zhong Y. Measurement of antioxidant activity. J Funct Foods. 2015;18:757–81.CrossRefGoogle Scholar
  15. 15.
    Assefa AD, Keum Y-S, Saini RK. A comprehensive study of polyphenols contents and antioxidant potential of 39 widely used spices and food condiments. J Food Meas Charact. 2018;12:1548–55.CrossRefGoogle Scholar
  16. 16.
    Ambigaipalan P, de Camargo AC, Shahidi F. Identification of phenolic antioxidants and bioactives of pomegranate seeds following juice extraction using HPLC-DAD-ESI-MS(n). Food Chem. 2017;221:1883–94.CrossRefGoogle Scholar
  17. 17.
    Sentkowska A, Pyrzynska K. Investigation of antioxidant interaction between green tea polyphenols and acetaminophen using isobolographic analysis. J Pharm Biomed Anal. 2018;159:393–7.CrossRefGoogle Scholar
  18. 18.
    Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins BD. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006;19:669–75.CrossRefGoogle Scholar
  19. 19.
    Vignault A, Gonzalez-Centeno MR, Pascual O, Gombau J, Jourdes M, Moine V, et al. Chemical characterization, antioxidant properties and oxygen consumption rate of 36 commercial oenological tannins in a model wine solution. Food Chem. 2018;268:210–9.CrossRefGoogle Scholar
  20. 20.
    Ioannone F, Di Mattia CD, De Gregorio M, Sergi M, Serafini M, Sacchetti G. Flavanols, proanthocyanidins and antioxidant activity changes during cocoa (Theobroma cacao L.) roasting as affected by temperature and time of processing. Food Chem. 2015;174:256–62.CrossRefGoogle Scholar
  21. 21.
    Floegel A, Kim D-O, Chung S-J, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal. 2011;24:1043–8.CrossRefGoogle Scholar
  22. 22.
    Elias RJ, Andersen ML, Skibsted LH, Waterhouse AL. Identification of free radical intermediates in oxidized wine using electron paramagnetic resonance spin trapping. J Agric Food Chem. 2009;57:4359–65.CrossRefGoogle Scholar
  23. 23.
    Bartoš J, Švajdlenková H, Zaleski R, Edelmann M, Lukešová M. Spin probe dynamics in relation to free volume in crystalline organics by means of ESR and PALS: n-hexadecane. Phys B Condens Matter. 2013;430:99–105.CrossRefGoogle Scholar
  24. 24.
    Weil J. A review of electron spin spectroscopy and its application to the study of paramagnetic defects in crystalline quartz. Phys Chem Miner. 1984;10:149–65.CrossRefGoogle Scholar
  25. 25.
    Minakata K, Suzuki O. Quantitation of manganese by use of an electron spin resonance method. Anal Chem. 2002;74:6111–3.CrossRefGoogle Scholar
  26. 26.
    Morsy MA, Sultan SM, Dafalla H. Electron paramagnetic resonance method for the quantitative assay of ketoconazole in pharmaceutical preparations. Anal Chem. 2009;81:6991–5.CrossRefGoogle Scholar
  27. 27.
    Borbat PP, Costa-Filho AJ, Earle KA, Moscicki JK, Freed JH. Electron spin resonance in studies of membranes and proteins. Science. 2001;291:266–9.CrossRefGoogle Scholar
  28. 28.
    Webb MI, Walsby CJ. EPR as a probe of the intracellular speciation of ruthenium(III) anticancer compounds. Metallomics. 2013;5:1624–33.CrossRefGoogle Scholar
  29. 29.
    Polovka M. EPR spectroscopy a tool to characterize stability. J Food Nutr Res. 2006;45:1–11.Google Scholar
  30. 30.
    Escudero R, Segura J, Velasco R, Valhondo M, Romero de Avila MD, Garcia-Garcia AB, et al. Electron spin resonance (ESR) spectroscopy study of cheese treated with accelerated electrons. Food Chem. 2019;276:315–21.CrossRefGoogle Scholar
  31. 31.
    Tian S, Jiang J, Zang S, Wang K, Yu Y, Li X, et al. Determination of IgG by electron spin resonance spectroscopy using Fe3O4 nanoparticles as probe. Microchem J. 2018;141:444–50.CrossRefGoogle Scholar
  32. 32.
    Jiang J, Tian S, Wang K, Wang Y, Zang S, Yu A, et al. Electron spin resonance spectroscopy for immunoassay using iron oxide nanoparticles as probe. Anal Bioanal Chem. 2018;410:1817–24.CrossRefGoogle Scholar
  33. 33.
    Bartoszek M, Polak J. A comparison of antioxidative capacities of fruit juices, drinks and nectars, as determined by EPR and UV-vis spectroscopies. Spectrochim Acta A Mol Biomol. 2016;153:546–9.CrossRefGoogle Scholar
  34. 34.
    Zang S, Tian S, Jiang J, Han D, Yu X, Wang K, et al. Determination of antioxidant capacity of diverse fruits by electron spin resonance (ESR) and UV-vis spectrometries. Food Chem. 2017;221:1221–5.CrossRefGoogle Scholar
  35. 35.
    Azman NA, Peiro S, Fajari L, Julia L, Almajano MP. Radical scavenging of white tea and its flavonoid constituents by electron paramagnetic resonance (EPR) spectroscopy. J Agric Food Chem. 2014;62:5743–8.CrossRefGoogle Scholar
  36. 36.
    Polak J, Bartoszek M, Stanimirova I. A study of the antioxidant properties of beers using electron paramagnetic resonance. Food Chem. 2013;141:3042–9.CrossRefGoogle Scholar
  37. 37.
    Li D, Jiang J, Han D, Yu X, Wang K, Zang S, et al. Measurement of antioxidant capacity by electron spin resonance spectroscopy based on copper(II) reduction. Anal Chem. 2016;88:3885–90.CrossRefGoogle Scholar
  38. 38.
    Jiang J, Zang S, Li D, Wang K, Tian S, Yu A, et al. Determination of antioxidant capacity of thiol-containing compounds by electron spin resonance spectroscopy based on Cu2+ ion reduction. Talanta. 2018;184:23–8.CrossRefGoogle Scholar
  39. 39.
    Apak R, Güçlü K, Özyürek M, Çelik SE. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchim Acta. 2007;160:413–9.CrossRefGoogle Scholar
  40. 40.
    Sellappan S, Akoh C, Krewer G. Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries. J Agric Food Chem. 2002;50:2432–8.CrossRefGoogle Scholar
  41. 41.
    Özyürek M, Güçlü K, Apak R. The main and modified CUPRAC methods of antioxidant measurement. Trends Analyt Chem. 2011;30:652–64.CrossRefGoogle Scholar
  42. 42.
    Piljac-Žegarac J, Valek L, Martinez S, Belščak A. Fluctuations in the phenolic content and antioxidant capacity of dark fruit juices in refrigerated storage. Food Chem. 2009;113:394–400.CrossRefGoogle Scholar
  43. 43.
    Locatelli M, Gindro R, Travaglia F, Coïsson J-D, Rinaldi M, Arlorio M. Study of the DPPH-scavenging activity: development of a free software for the correct interpretation of data. Food Chem. 2009;114:889–97.CrossRefGoogle Scholar
  44. 44.
    Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem. 1998;46:4113–7.CrossRefGoogle Scholar
  45. 45.
    Vasco C, Ruales J, Kamal-Eldin A. Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chem. 2008;111:816–23.CrossRefGoogle Scholar
  46. 46.
    Fu L, Xu BT, Xu XR, Gan RY, Zhang Y, Xia EQ, et al. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem. 2011;129:345–50.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Sizhu Tian
    • 1
  • Xuwen Li
    • 1
  • Jia Jiang
    • 1
  • Kun Wang
    • 1
  • Hanqi Zhang
    • 1
  • Aimin Yu
    • 1
  • Ziwei Zhang
    • 1
    Email author
  1. 1.College of ChemistryJilin UniversityChangchunChina

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