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Black oxidized 3,3′,5,5′-tetramethylbenzidine nanowires (oxTMB NWs) for enhancing Pt nanoparticle-based strip immunosensing

  • Shan Lin
  • Danmin Zheng
  • Ailing Li
  • Yuwu ChiEmail author
Paper in Forefront
  • 82 Downloads
Part of the following topical collections:
  1. New Insights into Analytical Science in China

Abstract

It is well known that 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized into blue or yellow oxidzed TMB (oxTMB) with the catalysis of peroxidase or mimetic enzyme of platinum nanoparticles (Pt NPs). In this work, we found that TMB could be oxidized into very stable black oxTMB with the catalysis of Pt NPs under certain chromogenic reaction conditions. For the first time, the black oxTMB was revealed to consist of nanowires (oxTMB NWs) with lengths of more than 100 μm and diameters of around 100 nm. The black oxTMB NWs showed very strong light absorption ability, thus could be used to greatly amplify the signal of Pt NP-based immunochromatography test strips (ICTSs). The Pt NP-based ICTSs with black oxTMB NW signal amplification have shown much better assay ability (linear response range and limit of detection) than those of gold nanoparticle (Au NP)-based ICTS, Pt NP-based ICTS, and Pt NP-based ICTS with blue or yellow oxTMB signal amplifications. The developed Pt NP-oxTMB NW-based ICTS has been demonstrated to be a new, accurate, sensitive, selective, and rapid immunosensor for quantitative detection of antigens such as human chorionic gonadotropin (HCG).

Keywords

Immunochromatography test strip (ICTS) Human chorionic gonadotropin (HCG) 3,3′,5,5′-Tetramethylbenzidine (TMB) Nanowires Platinum nanoparticles 

Notes

Funding information

This work was financially supported by the National Natural Science Foundation of China (21675027), the Program for Scientific and Technological Innovation Leading Talents in Fujian Province, and the Program for Changjiang Scholars and Innovative Research Team in University (No.IRT_15R11).

Compliance with ethical standards

All experiments were approved by the Ethics Committee at Fujian Medical University Union Hospital and performed in accordance with the ethical standards. Informed consents were obtained from all individual participants in accordance with the guidelines for conducting the clinical research.

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2019_1745_MOESM1_ESM.pdf (1.1 mb)
ESM 1 (PDF 1172 kb)

References

  1. 1.
    Wang K, Qin W, Hou Y, Xiao K, Yan W. The application of lateral flow immunoassay in point of care testing: a review. Nano Biomed Eng. 2016;8:172–83.CrossRefGoogle Scholar
  2. 2.
    Prasad PV, Chaube SK, Shrivastav TG, Kumari GL. Development of colorimetric enzyme-linked immunosorbent assay for human chorionic gonadotropin. J Immunoass Immunoch. 2006;27:15–30.CrossRefGoogle Scholar
  3. 3.
    Wada A, Sakoda Y, Oyamada T, Kida H. Development of a highly sensitive immunochromatographic detection kit for H5 influenza virus hemagglutinin using silver amplification. J Virol Methods. 2011;178:82–6.CrossRefGoogle Scholar
  4. 4.
    Wang J, Chen M, Sheng Z, Liu D, Wu S, Lai W. Development of colloidal gold immunochromatographic signal-amplifying system for ultrasensitive detection of Escherichia coli O157:H7 in milk. RSC Adv. 2015;5:62300–5.CrossRefGoogle Scholar
  5. 5.
    Jiang T, Song Y, Du D, Liu X, Lin Y. Detection of p53 protein based on mesoporous Pt-Pd nanoparticles with enhanced peroxidase-like catalysis. ACS sensors. 2016;1:717–24.CrossRefGoogle Scholar
  6. 6.
    Chen A, Holt-Hindle P. Platinum-based nanostructured materials: synthesis, properties, and applications. Chem Rev. 2010;110:3767–804.CrossRefGoogle Scholar
  7. 7.
    Fan J, Yin JJ, Ning B, Wu X, Hu Y, Ferrari M, et al. Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles. Biomaterials. 2011;32:1611–8.CrossRefGoogle Scholar
  8. 8.
    Gao Z, Xu M, Hou L, Chen G, Tang D. Irregular-shaped platinum nanoparticles as peroxidase mimics for highly efficient colorimetric immunoassay. Anal Chim Acta. 2013;776:79–86.CrossRefGoogle Scholar
  9. 9.
    Ju Y, Kim J. Dendrimer-encapsulated Pt nanoparticles with peroxidase-mimetic activity as biocatalytic labels for sensitive colorimetric analyses. Chem Commun. 2015;51:13752–5.CrossRefGoogle Scholar
  10. 10.
    Li W, Chen B, Zhang H, Sun Y, Wang J, Zhang J, et al. BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury (II) ions. Biosens Bioelectron. 2015;66:251–8.CrossRefGoogle Scholar
  11. 11.
    Liu J, Hu X, Hou S, Wen T, Liu W, Zhu X, et al. Au@Pt core/shell nanorods with peroxidase- and ascorbate oxidase-like activities for improved detection of glucose. Sensors Actuators B Chem. 2012:708–14.Google Scholar
  12. 12.
    Wang Z, Yang X, Yang J, Jiang Y, He N. Peroxidase-like activity of mesoporous silica encapsulated Pt nanoparticle and its application in colorimetric immunoassay. Anal Chim Acta. 2015;862:53–63.CrossRefGoogle Scholar
  13. 13.
    Josephy PD, Eling T, Mason RP. The horseradish peroxidase-catalyzed oxidation of 3,5,3’,5’-tetramethylbenzidine. J Biol Chem. 1982;257:3669–75.Google Scholar
  14. 14.
    Liu Y, Wu H, Li M, Yin J, Nie Z. pH dependent catalytic activities of platinum nanoparticles with respect to the decomposition of hydrogen peroxide and scavenging of superoxide and singlet oxygen. Nanoscale. 2014;6:11904–10.CrossRefGoogle Scholar
  15. 15.
    Jiang T, Song Y, Wei T, Li H, Du D, Zhu M, et al. Sensitive detection of Escherichia coli O157:H7 using Pt–Au bimetal nanoparticles with peroxidase-like amplification. Biosens Bioelectron. 2016;77:687–94.CrossRefGoogle Scholar
  16. 16.
    Zhang A, Guo W, Ke H, Zhang X, Zhang H, Huang C, et al. Sandwich-format ECL immunosensor based on Au star@BSA-Luminol nanocomposites for determination of human chorionic gonadotropin. Biosens Bioelectron. 2018;101:219–26.CrossRefGoogle Scholar
  17. 17.
    Lei J, Jing T, Zhou T, Zhou Y, Wu W, Mei S, et al. A simple and sensitive immunoassay for the determination of human chorionic gonadotropin by graphene-based chemiluminescence resonance energy transfer. Biosens Bioelectron. 2014;54:72–7.CrossRefGoogle Scholar
  18. 18.
    Kou B, Chai Y, Yuan Y, Yuan R. PtNPs as scaffolds to regulate interenzyme distance for construction of efficient enzyme cascade amplification for ultrasensitive electrochemical detection of MMP-2. Anal Chem. 2017;89:9383–7.CrossRefGoogle Scholar
  19. 19.
    Zhou J, Zhuang J, Miro M, Gao Z, Chen G, Tang D. Carbon nanospheres-promoted electrochemical immunoassay coupled with hollow platinum nanolabels for sensitivity enhancement. Biosens Bioelectron. 2012;35:394–400.CrossRefGoogle Scholar
  20. 20.
    Zhou C, Chen Y, Shang P, Chi Y. Strong electrochemiluminescent interactions between carbon nitride nanosheet-reduced graphene oxide nanohybrids and folic acid, and ultrasensitive sensing for folic acid. Analyst. 2016;141:3379–88.CrossRefGoogle Scholar
  21. 21.
    Vashist SK, Mudanyali O, Schneider EM, Zengerle R, Ozcan A. Cellphone–based devices for bioanalytical sciences. Anal Bioanal Chem. 2014;406:3263–77.CrossRefGoogle Scholar
  22. 22.
    Vashist SK, Luppa PB, Yeo LY, Ozcan A, Luong JHT. Emerging technologies for next–generation point-of-care testing. Trends Biotechnol. 2015;33:692–705.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory for Analytical Science of Food Safety and Biology, Ministry of Education, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and College of ChemistryFuzhou UniversityFuzhouChina

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