Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone
Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78–25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1–93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.
KeywordsLateral flow assay Fluorescent quenching Zearalenone Quantum dots
This work was supported Grant from the National Key Technology R & D Program, No. 2008BAK42B05 and Guangdong Province Key Scientific Research, No. 2013A022100031.
Compliance with ethical standards
Use of immune spleen cells for the production of monoclonal antibodies was approved by the Experimental Animal Ethics Committee of Jinan University. The Jinan University Experimental Animal Ethics Committee approved all animal experiments for preparation of anti-ZEN antibody and all experiments according to China Laboratory Animal Guidelines.
Conflicts of interest
There is no conflict of interest.
- 2.Li X, Li P, Zhang Q, Li R, Zhang W, Zhang Z, et al. Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B 1, ochratoxin A and zearalenone in agro-food. Biosens Bioelectron. 2013;49:426–32.Google Scholar
- 4.Organization WH (1981) Toxicological evaluation of certain food additives. In: Food Additives Series (FAO/WHO). vol 16. Geneva: World Health Organization.Google Scholar
- 5.Kovács F, Szathmáry C, Palyusik M. Data on determination of toxin F-2 (zearalenone) by high-pressure liquid, gas and thin-layer chromatography. Acta Veter Acad Sci Hung. 1975;25(2–3):223.Google Scholar
- 6.Soares LM, Rodriguez-Amaya DB. Survey of aflatoxins, ochratoxin A, zearalenone, and sterigmatocystin in some Brazilian foods by using multi-toxin thin-layer chromatographic method. J Assoc Off Anal Chem. 1989;72(1):22–6.Google Scholar
- 8.Wang Y, Chai T, Lu G, Quan C, Duan H, Yao M, et al. Simultaneous detection of airborne aflatoxin, ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography. Environ Res. 2008;107(2):139–44.Google Scholar
- 10.Bennett GA, Nelsen TC, Miller BM. Enzyme-linked immunosorbent assay for detection of zearalenone in corn, wheat, and pig feed: collaborative study. J AOAC Int. 1994;77(6):1500–9.Google Scholar
- 12.Chen Y, Fu Q, Li D, Xie J, Ke D, Song Q, et al. A smartphone colorimetric reader integrated with an ambient light sensor and a 3D printed attachment for on-site detection of zearalenone. Anal Bioanal Chem. 2017;409(28):6567–74.Google Scholar
- 14.Sun Y, Xing G, Yang J, Wang F, Deng R, Zhang G, Zhang Y. Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. J Sci Food Agric. 2016;96(11):3673–8.Google Scholar
- 15.Fu Q, Liu HL, Wu Z, Liu A, Yao C, Li X, et al. Rough surface Au@ Ag core–shell nanoparticles to fabricating high sensitivity SERS immunochromatographic sensors. J Nanobiotech. 2015;13(1):81.Google Scholar
- 17.Liang J, Liu H, Lan C, Fu Q, Huang C, Luo Z, et al. Silver nanoparticle enhanced Raman scattering-based lateral flow immunoassays for ultra-sensitive detection of the heavy metal chromium. Nanotechnology. 2014;25(49):495501.Google Scholar
- 19.Yeo SJ, Cuc BT, Kim SA, Kim DT, Bao DT, Tien TT, et al. Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye. Biosens Bioelectron. 2017;94:677.Google Scholar
- 21.Chen Y, Chen Q, Han M, Liu J, Zhao P, He L, et al. Near-infrared fluorescence-based multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk. Biosens Bioelectron. 2016;79:430–4.Google Scholar
- 23.Hu J, Zhang ZL, Wen CY, Tang M, Wu LL, Liu C, et al. Sensitive and quantitative detection of C-reaction protein based on immunofluorescent nanospheres coupled with lateral flow test strip. Anal Chem. 2016;88(12):6577.Google Scholar
- 26.Qu H, Zhang Y, Qu B, Kong H, Qin G, Liu S, et al. Rapid lateral-flow immunoassay for the quantum dot-based detection of puerarin. Biosens Bioelectron. 2016;81:358–62.Google Scholar
- 27.Li Y, Teng J, Zhu M, Lei Z, Zhong Y, Liu G, et al. MWCNTs based high sensitive lateral flow strip biosensor for rapid determination of aqueous mercury ions. Biosens Bioelectron. 2016;85:331.Google Scholar
- 28.Nan C, Ying S, Xu Y, Li Z, Luo Y, Huang K, et al. On-site detection of stacked genetically modified soybean based on event-specific TM-LAMP and a DNAzyme-lateral flow biosensor. Biosens Bioelectron. 2016;91:408–16.Google Scholar
- 29.Jiang T, Song Y, Du D, Liu X, Lin Y. Detection of p53 protein based on sesoporous Pt–Pd nanoparticles with enhanced peroxidase-like catalysis. ACS Sens. 2016;(6):717–24.Google Scholar
- 32.Teshima R, Kawase M, Tanaka T, Hirai K, Sato M, Sawada J, et al. Production and characterization of a specific monoclonal antibody against mycotoxin zearalenone. Curr Stud Hematol Blood Transfus. 1990;66(58):115.Google Scholar