Analytical and Bioanalytical Chemistry

, Volume 411, Issue 10, pp 2169–2175 | Cite as

Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone

  • Yuan Chen
  • Qiangqiang Fu
  • Jun Xie
  • Hong WangEmail author
  • Yong TangEmail author
Research Paper


Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78–25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1–93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.


Lateral flow assay Fluorescent quenching Zearalenone Quantum dots 



This work was supported Grant from the National Key Technology R & D Program, No. 2008BAK42B05 and Guangdong Province Key Scientific Research, No. 2013A022100031.

Compliance with ethical standards

Use of immune spleen cells for the production of monoclonal antibodies was approved by the Experimental Animal Ethics Committee of Jinan University. The Jinan University Experimental Animal Ethics Committee approved all animal experiments for preparation of anti-ZEN antibody and all experiments according to China Laboratory Animal Guidelines.

Conflicts of interest

There is no conflict of interest.

Supplementary material

216_2019_1652_MOESM1_ESM.pdf (292 kb)
ESM 1 (PDF 256 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Bioengineering, Guangdong Province Engineering Research Center for Antibody Drug and ImmunoassayJinan UniversityGuangzhouChina

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