Analytical and Bioanalytical Chemistry

, Volume 411, Issue 10, pp 2089–2099 | Cite as

A new SE-HPLC method for simultaneous quantification of proteins and main phenolic compounds from sunflower meal aqueous extracts

  • Sara Albe Slabi
  • Christelle Mathé
  • Xavier Framboisier
  • Claire Defaix
  • Odile Mesieres
  • Olivier Galet
  • Romain KapelEmail author
Research Paper


The aim of this research was to develop a method for simultaneous quantification of proteins and main polyphenolic compounds extracted from oleaginous meal by aqueous media. Size exclusion chromatography with a Biosep column (exclusion range from 1 to 300 kDa) and acetonitrile/water/formic acid (10:89.9:0.1 v/v) eluent at 0.6 mL min−1 yielded the most efficient separation of sunflower proteins and chlorogenic acid monoisomers (3-caffeoylquinic acid, 5-caffeoylquinic acid, and 4-caffeoylquinic acid). After a study of the stability of the extract components, the incorporation of a stabilization buffer (0.5 mol L−1 tris(hydroxymethyl)aminomethane-hydrochloric acid/1.0 mol L−1 sodium chloride at pH 7) was proposed to avoid polyphenol-protein interactions and/or isomeric transformation. The use of 214 nm as the wavelength for protein quantification was also included to minimize the effect of interference from polyphenol-protein interactions on the quantification. Under the used experimental conditions, the protein and chlorogenic acid monoisomer signals remained stable during 300 min at 20 °C (95–125% of the starting value). The developed method was validated and parameters such as specificity, sensitivity, precision, and accuracy were determined. The results from size exclusion chromatography correlated well with the results of protein determination by the reference Kjeldahl method. The proposed method was successfully applied for rapeseed extract analysis making simultaneous quantification of proteins and major rapeseed polyphenols (sinapine and sinapic acid) possible.

Graphical abstract


Size exclusion chromatography Simultaneous quantification Proteins Sunflower Chlorogenic acid Phenolic compounds 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human/animal rights

This article does not contain any studies with human or animal subjects performed by the any of the authors.

Supplementary material

216_2019_1635_MOESM1_ESM.pdf (350 kb)
ESM 1 (PDF 350 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Sara Albe Slabi
    • 1
    • 2
  • Christelle Mathé
    • 1
  • Xavier Framboisier
    • 1
  • Claire Defaix
    • 1
    • 2
  • Odile Mesieres
    • 1
  • Olivier Galet
    • 2
  • Romain Kapel
    • 1
    Email author
  1. 1.Laboratoire Réactions et Génie des ProcédésUniversité de Lorraine, CNRS, LRGPNancyFrance
  2. 2.Avril GroupParisFrance

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