Analytical and Bioanalytical Chemistry

, Volume 411, Issue 6, pp 1229–1238 | Cite as

Aptamer-mediated colorimetric and electrochemical detection of Pseudomonas aeruginosa utilizing peroxidase-mimic activity of gold NanoZyme

  • Ritu Das
  • Abhijeet Dhiman
  • Arti Kapil
  • Vipul BansalEmail author
  • Tarun Kumar SharmaEmail author
Research Paper


Despite of various advancements in biosensing, a rapid, accurate, and on-site detection of a bacterial pathogen is a real challenge due to the lack of appropriate diagnostic platforms. To address this unmet need, we herein report an aptamer-mediated tunable NanoZyme sensor for the detection of Pseudomonas aeruginosa, an infectious bacterial pathogen. Our approach exploits the inherent peroxidase-like NanoZyme activity of gold nanoparticles (GNPs) in combination with high affinity and specificity of a Pseudomonas aeruginosa–specific aptamer (F23). The presence of aptamer inhibits the inherent peroxidase-like activity of GNPs by simple adsorption on to the surface of GNPs. However, in the presence of cognate target (P. aeruginosa), owing to the high affinity for P. aeruginosa, the aptamer leaves the GNP surface, allowing GNPs to resume their peroxidase-like activity, resulting in oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). As TMB is an electrochemically active species, we have been able to translate the NanoZyme-based method into an ultrasensitive electrochemical assay using disposable carbon screen-printed electrode. This approach is highly sensitive and allows us to rapidly detect P. aeruginosa with a low-end detection limit of ~ 60 CFU/mL in water within 10 min. This generic aptamer-NanoZyme-based electrochemical sensing strategy may, in principle, be applicable for the detection of various other bacterial pathogens.


Aptamer Pseudomonas aeruginosa NanoZyme Colorimetric assay Electrochemical sensing 



The authors acknowledge the generous support provided by Prof. Jaya S. Tyagi and Dr. H.K. Prasad in providing laboratory access to handle the bacterial cultures.

Funding information

T.K.S. is thankful to THSTI Core grant and the Department of Biotechnology Govt. of India for Innovation Award and Innovative Young Biotechnologist Award (BT/010/IYBA/2016/10). V.B. thanks the Australian Research Council (ARC) for a Future Fellowship (FT140101285) and research support through an ARC Discovery (DP170103477) grant. V.B. also recognizes the generous support of the Ian Potter Foundation toward establishing the Sir Ian Potter NanoBioSensing Facility at RMIT University. A.D. is thankful to the Indian Council for Medical Research for providing Senior Research Fellowship.

Compliance with ethical standards

This research does not involve any human participant or animals.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_1555_MOESM1_ESM.pdf (613 kb)
ESM 1 (PDF 613 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.AptaBharat Innovation Pvt. Ltd.Interim Incubator of Translational Health Science and Technology Institute (THSTI)FaridabadIndia
  2. 2.Indian Institute of Technology-Delhi (IIT-Delhi)New DelhiIndia
  3. 3.Department of BiotechnologyAll India Institute of Medical Sciences (AIIMS)New DelhiIndia
  4. 4.Faculty of PharmacyUttarakhand Technical University (UTU)DehradunIndia
  5. 5.Department of MicrobiologyAll India Institute of Medical Sciences (AIIMS)New DelhiIndia
  6. 6.Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Lab (NBRL), School of ScienceRMIT UniversityMelbourneAustralia
  7. 7.Centre for Biodesign and DiagnosticsTranslational Health Science and Technology Institute (THSTI)FaridabadIndia

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