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Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat

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Abstract

Fluorescence polarization immunoassays (FPIAs) for thiabendazole and tetraconazole were first developed. Tracers for FPIAs of thiabendazole and tetraconazole were synthesized and the tracers’ structures were confirmed by HPLC-MS/MS. The 4-aminomethylfluorescein-labeled tracers allowed achieving the best assay sensitivity and minimum reagent consumption in comparison with aminofluorescein-labeled and alkyldiaminefluoresceinthiocarbamyl-labeled tracers. Measurements of fluorescence polarization were performed using a portable device. The developed FPIA methods were applied for the analysis of wheat. Fast and simple sample preparation technique earlier developed by authors for pesticides was adapted for thiabendazole and tetraconazole. The limits of detection of thiabendazole and tetraconazole in wheat were 20 and 200 μg/kg, and the lower limits of quantification were 40 and 600 μg/kg, respectively. The recovery test was performed by two methods—FPIA and HPLC-MS/MS. The results obtained by FPIA correlated well with those obtained by HPLC-MS/MS (r2 = 0.9985 for thiabendazole, r2 = 0.9952 for tetraconazole). Average recoveries of thiabendazole and tetraconazole were 74 ± 4% and 72 ± 3% by FPIA, and average recoveries of thiabendazole and tetraconazole were 86 ± 2% and 74 ± 1% by HPLC-MS/MS (n = 15).

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Abbreviations

(CH2)4DF:

Butylenediaminefluoresceinthiocarbamyl

(CH2)6DF:

Hexamethylenediaminefluoresceinthiocarbamyl

AF:

Aminofluorescein

AMF:

4-Aminomethylfluorescein

BSA:

Bovine serum albumin

CR:

Cross-reactivity

DCC:

Dicyclohexylcarbodiimide

EDF:

Ethylenediaminefluoresceinthiocarbamyl

ELISA:

Enzyme-linked immunosorbent assay

FI:

Fluorescence intensity

FITC:

Fluorescein isothiocyanate isomer I

FPIA:

Fluorescence polarization immunoassay

HPLC-MS/MS:

High-performance liquid chromatography coupled with tandem mass spectrometry

LOD:

Limit of detection

MRL:

Maximum residue level

NHS:

N-hydroxysuccinimide

SD:

Standard deviation

TLC:

Thin-layer chromatography

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Acknowledgments

The authors are thankful to Dr. A.V. Zherdev and Dr. E.A. Zvereva (Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences) for useful discussion of the obtained results.

Funding

The work was financially supported by the Russian Science Foundation (project No. 14-16-00149).

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Authors and Affiliations

  1. Faculty of Chemistry, Department of Chemical Enzymology, M.V. Lomonosov Moscow State University, Leninsky Gory 1, Moscow, Russia, 119991

    Anna Yu. Boroduleva & Sergei A. Eremin

  2. Centro de Investigación e Innovación en Bioingeniería (Ci2B), Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain

    Juan J. Manclús & Ángel Montoya

  3. A.N. Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Leninsky prospect 33, Moscow, Russia, 119071

    Sergei A. Eremin

Authors
  1. Anna Yu. Boroduleva
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  2. Juan J. Manclús
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  3. Ángel Montoya
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  4. Sergei A. Eremin
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Correspondence to Sergei A. Eremin.

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Boroduleva, A.Y., Manclús, J.J., Montoya, Á. et al. Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat. Anal Bioanal Chem 410, 6923–6934 (2018). https://doi.org/10.1007/s00216-018-1296-z

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  • DOI: https://doi.org/10.1007/s00216-018-1296-z

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