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Analyte-driven self-assembly of graphene oxide sheets onto hydroxycamptothecin-functionalized upconversion nanoparticles for the determination of type I topoisomerases in cell extracts

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Abstract

Type I topoisomerases (TOPOI), a potential diagnostic biomarker and a target for chemotherapeutic agents, play essential roles in DNA replication, transcription, chromosome segregation, and recombination. It is essential to develop analytical methods for accurate detection of TOPOI in biological fluids for early diagnosis of diseases. Here we show an assay for TOPOI on the basis of the target-induced self-assembly of graphene oxide (GO) sheets onto hydroxycamptothecin-functionalized upconversion nanoparticles (HCPT-UCNPs). The dipole-dipole coupling of HCPT-UCNPs (donor) and GO (acceptor) regulated by TOPOI enables Förster resonance energy transfer between the donor and the acceptor. Integration of minimal autofluorescence and highly specific affinity into the developed nanosensor allows reliable detection of TOPOI in the nanomolar range with the detection limit of 0.29 nM. The detection of TOPOI in breast cancer cells with recoveries from 96.3 to 103.7% shows the availability of the proposed assay in complicated samples.

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Funding

This study received financial support from the National Natural Science Foundation of China (Nos. 21435001 and 81401471), the China Postdoctoral Science Foundation (No. 2015 M581306), the Natural Science Foundation of Tianjin (No. 17JCYBJC20700), the Natural Science Foundation of Tianjin Medical University (No. 2014KYQ14), and the National First-class Discipline Program of Food Science and Technology (No. JUFSTR20180301).

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Authors and Affiliations

  1. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300072, China

    Xu Wang

  2. State Key Laboratory of Food Science and Technology, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Xiu-Ping Yan

Authors
  1. Xu Wang
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Correspondence to Xiu-Ping Yan.

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The authors declare that they have no conflict of interest.

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Published in the topical collection New Insights into Analytical Science in China with guest editors Lihua Zhang, Hua Cui, and Qiankun Zhuang.

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Wang, X., Yan, XP. Analyte-driven self-assembly of graphene oxide sheets onto hydroxycamptothecin-functionalized upconversion nanoparticles for the determination of type I topoisomerases in cell extracts. Anal Bioanal Chem 410, 6761–6769 (2018). https://doi.org/10.1007/s00216-018-1234-0

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  • DOI: https://doi.org/10.1007/s00216-018-1234-0

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