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Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials

Analytical and Bioanalytical Chemistry volume 410, pages 6067–6077 (2018)Cite this article

Abstract

The widespread use of engineered nanomaterials or nanotechnology makes the characterization of biological responses to nanomaterials an important area of research. The application of omics approaches, such as mass spectrometry-based proteomics, has revealed new insights into the cellular responses of exposure to nanomaterials, including how nanomaterials interact and alter cellular pathways. In addition, exposure to engineered nanomaterials often leads to the generation of reactive oxygen species and cellular oxidative stress, which implicates a redox-dependent regulation of cellular responses under such conditions. In this review, we discuss quantitative proteomics-based approaches, with an emphasis on redox proteomics, as a tool for system-level characterization of the biological responses induced by engineered nanomaterials.

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Funding

Portions of the work were supported by the National Institutes of Health Grants U01ES027292, a member of the Nanotechnology Health Implications Research (NHIR) consortium, and P41GM103493. The experimental work described herein was performed in the Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, a national scientific user facility sponsored by the Department of Energy under Contract DE-AC05-76RL0 1830.

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  1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA

    Tong Zhang, Matthew J. Gaffrey, Brian D. Thrall & Wei-Jun Qian

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  1. Tong Zhang
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  2. Matthew J. Gaffrey
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Correspondence to Wei-Jun Qian.

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Published in the topical collection Analytical Developments in Advancing Safety in Nanotechnology with guest editors Lisa Holland and Wenwan Zhong.

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Zhang, T., Gaffrey, M.J., Thrall, B.D. et al. Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials. Anal Bioanal Chem 410, 6067–6077 (2018). https://doi.org/10.1007/s00216-018-1168-6

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  • DOI: https://doi.org/10.1007/s00216-018-1168-6

Keywords

  • Engineered nanomaterials
  • Proteomics
  • Post-translational modifications
  • Redox proteomics
  • Thiol
  • Oxidative stress