Modification of polydopamine-coated Fe3O4 nanoparticles with multi-walled carbon nanotubes for magnetic-μ-dispersive solid-phase extraction of antiepileptic drugs in biological matrices
- 7 Downloads
In this study, multi-walled carbon nanotubes were coated on the surface of magnetic nanoparticles modified by polydopamine. The synthesized composite was characterized and applied to magnetic-μ-dispersive solid-phase extraction of oxcarbazepine (OXC), phenytoin (PHT), and carbamazepine (CBZ) from human plasma, urine, and cerebrospinal fluid samples prior to analysis by a high-performance liquid chromatography-photodiode array detector. The extraction parameters were investigated and the optimum condition was obtained when the variables were set to the following: sorbent type, Fe3O4@polyDA–MWCNTs (length < 2 μm); sample pH, 6; amount of sorbent, 15 mg; sorption time, 1.5 min at room temperature; type and volume of the eluent, 2.5 mL methanol; and salt content, none added. Under the optimized conditions, the calibration curves are linear in the concentration range 2–2000 ng/mL, the limits of detection are in the range 0.4–3.1 ng/mL, and the relative standard deviations and relative recoveries of plasma (spiked at 200 ng/mL) and CSF (spiked at 50 ng/mL) are in the ranges 1.4–8.2% and 92.8–96.5%, respectively. The applicability of the method was successfully confirmed by extraction and determination of OXC, PHT, and CBZ in biological matrices.
KeywordsAntiepileptic drugs Polydopamine Magnetic multi-walled carbon nanotube Biological matrices
Compliance with ethical standards
This is not a clinical study on humans with an ethics committee. Voluntary donors donate biological samples to the Peking Union Medical College Hospital and the samples were transferred to our laboratory. The Peking Union Medical College Hospital ensures donor-product-laboratory traceability and donor consent.
Conflict of interest
The authors declare that they have no competing interests.
- 8.Arghavani-Beydokhti S, Rajabi M, Asghari A. Combination of magnetic dispersive micro solid-phase extraction and supramolecular solvent-based microextraction followed by high-performance liquid chromatography for determination of trace amounts of cholesterol-lowering drugs in complicated matrices. Anal Bioanal Chem. 2017;409(18):1–13.CrossRefGoogle Scholar
- 9.Kong XJ, Zheng C, Lan YH, Chi SS, Dong Q, Liu HL, et al. Synthesis of multirecognition magnetic molecularly imprinted polymer by atom transfer radical polymerization and its application in magnetic solid-phase extraction. Anal Bioanal Chem. 2017;410(1):247–57.Google Scholar
- 10.Ghorbanil M, Chamsazl M, Rounaghil GH, Aghamohammadhasani M, Seyedin O, Lahoori NA. Development of a novel ultrasonic-assisted magnetic dispersive solid-phase microextraction method coupled with high performance liquid chromatography for determination of mirtazapine and its metabolites in human urine and water samples employing experimental design. Anal Bioanal Chem. 2016;408:7719–29.CrossRefGoogle Scholar
- 11.Corps Ricardo AI, Guzmán Bernardo FJ, Zougagh M, Rodríguez Martín-Doimeadios RC, Ríos A. Magnetic nanoparticles-carbon nanotubes hybrid composites for selective solid-phase extraction of polycyclic aromatic hydrocarbons and determination by ultra-high performance liquid chromatography. Anal Bioanal Chem. 2017;409(21):5125–32.CrossRefGoogle Scholar
- 17.Xiao D, Dramou P, Xiong N, He H, Li H, Yuan D, et al. Development of novel molecularly imprinted magnetic solid-phase extraction materials based on magnetic carbon nanotubes and their application for the determination of gatifloxacin in serum samples coupled with high performance liquid chromatography. J Chromatogr A. 2013;1274:44–53.Google Scholar
- 20.Morales-Cid G, Fekete A, Simonet BM, Lehmann R, Cardenas SC, Zhang X, et al. In situ synthesis of magnetic multiwalled carbon nanotube composites for the clean-up of (fluoro)quinolones from human plasma prior to ultrahigh pressure liquid chromatography analysis. Anal Chem. 2010;82:2743–52.Google Scholar
- 21.Zhu J, Wei S, Gu H, Rapole SB, Wang Q, Luo Z, et al. One-pot synthesis of magnetic graphene nanocomposites decorated with core@double-shell nanoparticles for fast chromium removal. Environ Sci Technol. 2012;46(2):977–85.Google Scholar
- 29.Che D, Cheng J, Ji Z, Zhang S, Li G, Sun Z, et al. Recent advances and applications of polydopamine-derived adsorbents for sample pretreatment. Trends Anal Chem. 2017;97:1–14.Google Scholar
- 37.Wang L, Wang J, Zhang J, Jiang Q, Zhao L, Zhang T. Simultaneous determination of topiramate, carbamazepine, oxcarbazepine and its major metabolite in human plasma by SFC-ESI-MS/MS with polarity switching: application to therapeutic drug monitoring. Arab J Chem. 2016; https://doi.org/10.1016/j.arabjc.2016.09.016.
- 38.Fortuna A, Sousa J, Alves G, Falcao A, Soares-da-Silva P. Development and validation of an HPLC-UV method for the simultaneous quantification of carbamazepine, oxcarbazepine, eslicarbazepine acetate and their main metabolites in human plasma. Anal Bioanal Chem. 2010;397(4):1605–15.CrossRefGoogle Scholar
- 39.Ferreira A, Rodrigues M, Oliveira P, Francisco J, Fortuna A, Rosado L, et al. Liquid chromatographic assay based on microextraction by packed sorbent for therapeutic drug monitoring of carbamazepine, lamotrigine, oxcarbazepine, phenobarbital, phenytoin and the active metabolites carbamazepine-10,11-epoxide and licarbazepine. J Chromatogr B. 2014;971:20–9.Google Scholar
- 40.Zhang J, Liu D, Meng X, Shi Y, Wang R, Xiao D, et al. Solid phase extraction based on porous magnetic graphene oxide/β-cyclodextrine composite coupled with high performance liquid chromatography for determination of antiepileptic drugs in plasma samples. J Chromatogr A. 2017;1524:49–56.Google Scholar