Analytical and Bioanalytical Chemistry

, Volume 410, Issue 17, pp 4051–4060 | Cite as

Proteomic approaches beyond expression profiling and PTM analysis

  • Jiaqi Fu
  • Mei Wu
  • Xiaoyun Liu


Essentially, all cellular functions are executed by proteins. Different physiological and pathological conditions dynamically control various properties of proteins, including expression levels, post-translational modifications (PTMs), protein–protein interactions, enzymatic activity, etc. Thus far, the vast majority of proteomic efforts have been focused on quantitative profiling of protein abundance/expression and their PTMs. In this article, we review some recent exciting progress in the development of proteomic approaches to examine protein functions from perspectives other than expression levels and PTMs. Specifically, we discuss advancements in proximity-based labeling, analysis of protein termini and newly synthesized proteins, and activity-based protein profiling.


Proximity-based labeling Terminal proteomics Newly synthesized proteins Activity-based protein profiling 



This work was financially supported by grants from the National Natural Science Foundation of China (21475005 and 21622501), Clinical Medicine Plus X-Young Scholars Project of Peking University and the Thousand Young Talents program of the Chinese government.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular EngineeringPeking UniversityBeijingChina

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