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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 12, pp 2981–2989 | Cite as

Fluorescent aptasensor for detection of four tetracycline veterinary drugs in milk based on catalytic hairpin assembly reaction and displacement of G-quadruplex

  • Chen Zhou
  • Haimin Zou
  • Chengjun Sun
  • Dongxia Ren
  • Wei Xiong
  • Yongxin Li
Research Paper

Abstract

Based on a novel signal amplification strategy by catalytic hairpin assembly and displacement of G-quadruplex DNA, an enzyme-free, non-label fluorescent aptasensing approach was established for sensitive detection of four tetracycline veterinary drugs in milk. The network consisted of a pair of partially complementary DNA hairpins (HP1 and HP2). The DNA aptamer of four tetracycline veterinary drugs was located at the sticky end of the HP1. The ring region of HP1 rich in G and C could form a stable G-quadruplex structure, which could emit specific fluorescence signal after binding with the fluorescent dye and N-methylmesoporphyrin IX (NMM). When presented in the system, the target analytes would be repeatedly used to trigger a recycling procedure between the hairpins, generating numerous HP1–HP2 duplex complexes and displacing G-quadruplex DNA. Thus, the sensitive detection of target analytes was achieved in a wide linear range (0–1000 μg/L) with the detection limit of 4.6 μg/L. Moreover, this proposed method showed high discrimination efficiency towards target analytes against other common mismatched veterinary drugs, and could be successfully applied to the analysis of milk samples.

Graphical abstract

Schematic of target analyte detection based on catalytic hairpin assembly reaction and displacement of G-quadruplex

Keywords

Tetracycline veterinary drugs Milk samples Fluorescence G-quadruplex N-methylmesoporphyrin IX Catalytic hairpin assembly 

Notes

Acknowledgments

The authors appreciate funding from China Postdoctoral Science Foundation (2016M590894) and the Fundamental Research Funds for the Central Universities (2012017yjsy207).

Compliance with ethical standards

Conflicts of interest

There are no conflicts of interest to declare.

Supplementary material

216_2018_981_MOESM1_ESM.pdf (142 kb)
ESM 1 (PDF 142 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chen Zhou
    • 1
  • Haimin Zou
    • 1
    • 2
  • Chengjun Sun
    • 1
  • Dongxia Ren
    • 1
  • Wei Xiong
    • 1
  • Yongxin Li
    • 1
    • 3
  1. 1.West China School of Public HealthSichuan UniversityChengduChina
  2. 2.Chengdu Center for Disease Control and PreventionChengduChina
  3. 3.College of Life SciencesSichuan UniversityChengduChina

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