Analytical and Bioanalytical Chemistry

, Volume 410, Issue 11, pp 2781–2791 | Cite as

Tuning of gold nanoclusters sensing applications with bovine serum albumin and bromelain for detection of Hg2+ ion and lambda-cyhalothrin via fluorescence turn-off and on mechanisms

  • Jigna R. Bhamore
  • Sanjay Jha
  • Hirakendu Basu
  • Rakesh Kumar Singhal
  • Z. V. P. Murthy
  • Suresh Kumar Kailasa
Research Paper


Herein, fluorescent gold nanoclusters (Au NCs) were obtained by one-pot synthetic method using bovine serum albumin (BSA) and bromelain as templates. As-synthesized fluorescent Au NCs were stable and showed bright red fluorescence under UV lamp at 365 nm. The fluorescent Au NCs exhibit the emission intensity at 648 nm when excited at 498 nm. Various techniques were used such as spectroscopy (UV-visible, fluorescence, and Fourier-transform infrared), high-resolution transmission electron microscopy, and dynamic light scattering for the characterization of fluorescent Au NCs. The values of I0/I at 648 nm are proportional to the concentrations of Hg2+ ion in the range from 0.00075 to 5.0 μM and of lambda-cyhalothrin in the range from 0.01 to 10 μM with detection limits of 0.0003 and 0.0075 μM for Hg2+ ion and lambda-cyhalothrin, respectively. The practical application of the probe was successfully demonstrated by analyzing Hg2+ ion and lambda-cyhalothrin in water samples. In addition, Au NCs used as probes for imaging of Simplicillium fungal cells. These results indicated that the as-synthesized Au NCs have proven to be promising fluorescent material for the sensing of Hg2+ ion and lambda-cyhalothrin in environmental and for imaging of microorganism cells in biomedical applications.


Au NCs BSA Bromelain Hg2+ ion Lambda-cyhalothrin Optical spectroscopy 



We gratefully acknowledge financial support by Department of Science and Technology, Government of India (EMR/2016/002621/IPC). Ms. Bhamore acknowledges the Director, SVNIT, Surat for financial support under the Doctoral Program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

216_2018_958_MOESM1_ESM.pdf (437 kb)
ESM 1 (PDF 425 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jigna R. Bhamore
    • 1
  • Sanjay Jha
    • 2
  • Hirakendu Basu
    • 3
  • Rakesh Kumar Singhal
    • 3
  • Z. V. P. Murthy
    • 4
  • Suresh Kumar Kailasa
    • 1
  1. 1.Department of Applied ChemistryS. V. National Institute of TechnologySuratIndia
  2. 2.Gujarat Agricultural Biotechnology InstituteNavsari Agricultural UniversitySuratIndia
  3. 3.Analytical Chemistry Division, Bhabha Atomic Research CenterMumbaiIndia
  4. 4.Chemical Engineering DepartmentS. V. National Institute of TechnologySuratIndia

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