Multi-layer solid-phase extraction and evaporation—enrichment methods for polar organic chemicals from aqueous matrices
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Analysis of polar organic chemicals in the aquatic environment is exacerbated by the lack of suitable and widely applicable enrichment methods. In this work, we assessed the suitability of a novel combination of well-known solid-phase extraction (SPE) materials in one cartridge as well as an evaporation method and for the enrichment of 26 polar model substances (predominantly log D < 0) covering a broad range of physico-chemical properties in three different aqueous matrices. The multi-layer solid-phase extraction (mlSPE) and evaporation method were investigated for the recovery and matrix effects of the model substances and analyzed with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). In total, 65% of the model substances were amenable (> 10% recovery) to the mlSPE method with a mean recovery of 76% while 73% of the model substances were enriched with the evaporation method achieving a mean recovery of 78%. Target and non-target screening comparison of both methods with a frequently used reversed-phase SPE method utilizing “hydrophilic and lipophilic balanced” (HLB) material was performed. Target analysis showed that the mlSPE and evaporation method have pronounced advantages over the HLB method since the HLB material retained only 30% of the model substances. Non-target screening of a ground water sample with the investigated enrichment methods showed that the median retention time of all detected features on a HILIC system decreased in the order mlSPE (3641 features, median tR 9.7 min), evaporation (1391, 9.3 min), HLB (4414, 7.2 min), indicating a higher potential of the described methods to enrich polar analytes from water compared with HLB-SPE.
KeywordsPolar organic chemicals Solid-phase extraction Sample preparation Non-target screening HILIC
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Conflict of interest
The authors declare that they have no conflict of interest.
- 4.Wirth O, Bunke D. PMT-Stoffe erkennen und ihre Emissionen vermeiden. https://www.umweltbundesamt.de/sites/default/files/medien/362/dokumente/2017_05_02_ridp_ws5_wasser_final.pdf. Last Access 07 2017.
- 11.Guillarme D, Veuthey J-L. Alternative strategies to reverse-phase liquid chromatography for the analysis of pharmaceutical compounds. Am Pharm Rev. 2017;20:46–51.Google Scholar
- 18.Huntscha S, Singer HP, McArdell CS, Frank CE, Hollender J. Multiresidue analysis of 88 polar organic micropollutants in ground, surface and wastewater using online mixed-bed multilayer solid-phase extraction coupled to high performance liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2012;1268:74–83.CrossRefGoogle Scholar
- 21.Young, MS. Solid-phase extraction with Oasis® HLB sorbent: simple procedures for superior sample preparation. Operating manual; 1998.Google Scholar
- 23.Chemicalize. Calculation. https://www.chemicalize.com. Accessed Nov 16 2017.
- 28.Liska I, Krupcík J, Leclercq PA. The use of solid sorbents for directaccumulation of organic compounds from water matricesa review of solid-phase extraction techniques. J High Resolut Chromatogr. 1989:577–90.Google Scholar