Analytical and Bioanalytical Chemistry

, Volume 410, Issue 19, pp 4567–4573 | Cite as

Advances in the analysis of biological samples using ionic liquids

  • Kevin D. Clark
  • María J. Trujillo-Rodríguez
  • Jared L. Anderson
Part of the following topical collections:
  1. Ionic Liquids as Tunable Materials in (Bio)Analytical Chemistry


Ionic liquids are a class of solvents and materials that hold great promise in bioanalytical chemistry. Task-specific ionic liquids have recently been designed for the selective extraction, separation, and detection of proteins, peptides, nucleic acids, and other physiologically relevant analytes from complex biological samples. To facilitate rapid bioanalysis, ionic liquids have been integrated in miniaturized and automated procedures. Bioanalytical separations have also benefited from the modification of nonspecific magnetic materials with ionic liquids or the implementation of ionic liquids with inherent magnetic properties. Furthermore, the direct detection of the extracted molecules in the analytical instrument has been demonstrated with structurally tuned ionic liquids and magnetic ionic liquids, providing a significant advantage in the analysis of low-abundance analytes. This article gives an overview of these advances that involve the application of ionic liquids and derivatives in bioanalysis.

Graphical abstract

Ionic liquids, magnetic ionic liquids, and ionic liquid-based sorbents are increasing the speed, selectivity, and sensitivity in the analysis of biological samples


Task-specific ionic liquid Magnetic ionic liquid Bioanalysis Automation Miniaturization Magnetic separation 



JLA acknowledges funding from the Chemical Measurement and Imaging Program at the National Science Foundation (grant no. CHE-1709372).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Kevin D. Clark
    • 1
  • María J. Trujillo-Rodríguez
    • 1
  • Jared L. Anderson
    • 1
  1. 1.Department of ChemistryIowa State UniversityAmesUSA

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