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Analytical and Bioanalytical Chemistry

, Volume 410, Issue 15, pp 3533–3545 | Cite as

Combined analytical approaches to define biodistribution and biological activity of semi-synthetic berberrubine, the active metabolite of natural berberine

  • Emanuele Porru
  • Placido Franco
  • Donato Calabria
  • Silvia Spinozzi
  • Marinella Roberti
  • Cristiana CalicetiEmail author
  • Aldo Roda
Research Paper
Part of the following topical collections:
  1. Discovery of Bioactive Compounds

Abstract

Berberine (BBR) is a natural alkaloid obtained from Berberis species plants, known for its protective effects against several diseases. Among the primary BBR metabolites, berberrubine (M1) showed the highest plasma concentration but few and conflicting data are available regarding its concentration in biological fluids related to its new potential activity on vascular cells. A combined analytical approach was applied to study biodistribution of M1 in comparison with BBR. The optimization of sample clean-up combined with a fully validated HPLC-ESI-MS/MS tailored for M1 allows sufficient detectability and accuracy to be reached in the different studied organs even when administered at low dose, comparable to that assumed by human. A predictive human vascular endothelial cell-based assay to measure intracellular xanthine oxidase has been developed and applied to study unexplored activities of M1 alongside other common activities. Results showed that oral M1 treatment exhibits higher plasma levels than BBR, reaching maximum concentration 400-fold higher than BBR (204 vs 0.5 ng/mL); moreover, M1 exhibits higher concentrations than BBR also in all the biological compartments analyzed. Noteworthy, the two compounds follow two different excretion routes: M1 through urine, while BBR through feces. In vitro studies demonstrated that M1 inhibited intracellular xanthine oxidase activity, one of the major sources of reactive oxygen species in vasculature, with an IC50 = 9.90 ± 0.01 μg/mL and reduced the expression of the inflammatory marker ICAM-1. These peculiar characteristics allow new perspectives to be opened up for the direct use of M1 instead of BBR in endothelial dysfunction treatment.

Keywords

Berberrubine Biodistribution Mass spectrometry Chemiluminescent cell-based assay Endothelial dysfunction Xanthine oxidase 

Notes

Compliance with ethical standards

All experiments were carried out according to the guidelines set forth by EEC Directive 86/609 on care and use of experimental animals. The protocol was approved by the Institutional Ethics Committee of the University of Bologna (Protocol 398/2016). All studies involving animals are reported in accordance with the ARRIVE guidelines.

Conflict of interest

The authors declare that they have no competing interest.

Supplementary material

216_2018_884_MOESM1_ESM.pdf (202 kb)
ESM 1 (PDF 202 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Emanuele Porru
    • 1
  • Placido Franco
    • 1
  • Donato Calabria
    • 2
  • Silvia Spinozzi
    • 1
  • Marinella Roberti
    • 3
  • Cristiana Caliceti
    • 1
    • 2
    • 4
    Email author
  • Aldo Roda
    • 1
    • 2
    • 4
  1. 1.Department of Chemistry “Giacomo Ciamician”University of BolognaBolognaItaly
  2. 2.Interdepartimental Center of Industrial Research (CIRI)-Energy and EnvironmentUniversity of BolognaBolognaItaly
  3. 3.Department of Pharmaceutical Sciences (FABIT)University of BolognaBolognaItaly
  4. 4.Istituto Nazionale Biostrutture e Biosistemi (INBB)RomeItaly

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