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Synthesis of multirecognition magnetic molecularly imprinted polymer by atom transfer radical polymerization and its application in magnetic solid-phase extraction

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Abstract

In this work, we reported an effective method for the synthesis of a multirecognition magnetic molecularly imprinted polymer (MMIP) with atom transfer radical polymerization (ATRP), using 2,4-diamino-6-methyl-1,3,5-triazine as pseudo-template. The resulting MMIP was characterized in detail by Fourier transform-infrared (FT-IR) spectra, scanning electron microscopy (SEM), thermogravimetic analysis (TGA), and vibrating sample magnetometry (VSM). These results indicated the successful synthesis of MMIP with sufficient thermal stability and magnetic properties. The adsorption experiments were carried out to evaluate the specific selectivity of MMIP related to the spatial structure of target molecules. The MMIP exhibited multirecognition ability and excellent binding capability for melamine (MEL), cyromazine (CYR), triamterene (TAT), diaveridine (DVD), and trimethoprim (TME), and the apparent maximum number of binding sites (Q max) was 77.5, 75.2, 72.5, 69.9, and 70.4 μmol g−1, respectively. The multirecognition MMIP not only possessed adequate magnetic responsiveness for fast separation but also avoided the risk of template leakage on trace component analysis. Therefore, it was suitable for serving as a magnetic solid-phase extraction (MSPE) adsorbent. MSPE coupled with high-performance liquid chromatography analysis was applied to enrich and separate five target molecules from three samples. Recoveries for all target molecules ranged from 81.6 to 91.5% with relative standard deviations of no more than 4.1% (n = 3).

Multirecognition property of magnetic molecularly imprinted polymer prepared with pseudo template

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 21605114, 21406103), National Student’s Platform for Innovation and Entrepreneurship Training Program (Grant No. 201610062011), China Postdoctoral Science Foundation (Grant No. 2015T80226), and Tianjin Natural Science Foundation (Grant No. 16JCQNJC05500).

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  1. Xiang-Jin Kong and Chao Zheng contributed equally to this work.

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Liaocheng University, Dongchangfu District, Liaocheng, Shandong, 252059, China

    Xiang-Jin Kong

  2. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, 22 Qixiangtai Road, Heping District, Tianjin, 300070, China

    Chao Zheng, Yao-Han Lan, Shuai-Shuai Chi, Qian Dong, Hao-Long Liu, Chao Peng, Lin-Yi Dong, Liang Xu & Xian-Hua Wang

  3. Tianjin Medical College, 14 Liulin Road, Hexi District, Tianjin, 300222, China

    Liang Xu

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Correspondence to Xian-Hua Wang.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of Tianjin Medical University and the Ethics Committee of Liaocheng University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Kong, XJ., Zheng, C., Lan, YH. et al. Synthesis of multirecognition magnetic molecularly imprinted polymer by atom transfer radical polymerization and its application in magnetic solid-phase extraction. Anal Bioanal Chem 410, 247–257 (2018). https://doi.org/10.1007/s00216-017-0716-9

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  • DOI: https://doi.org/10.1007/s00216-017-0716-9

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