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A highly selective electrochemical sensor based on molecularly imprinted polypyrrole-modified gold electrode for the determination of glyphosate in cucumber and tap water

Abstract

An electrochemical sensor based on molecularly imprinted polypyrrole (MIPPy) was developed for selective and sensitive detection of the herbicide glyphosate (Gly) in cucumber and tap water samples. The sensor was prepared via synthesis of molecularly imprinted polymers on a gold electrode in the presence of Gly as the template molecule and pyrrole as the functional monomer by cyclic voltammetry (CV). The sensor preparation conditions including the ratio of template to functional monomers, number of CV cycles in the electropolymerization process, the method of template removal, incubation time, and pH were optimized. Under the optimal experimental conditions, the DPV peak currents of hexacyanoferrate/hexacyanoferrite changed linearly with Gly concentration in the range from 5 to 800 ng mL−1, with a detection limit of 0.27 ng mL−1 (S/N = 3). The sensor was used to detect the concentration of Gly in cucumber and tap water samples, with recoveries ranging from 72.70 to 98.96%. The proposed sensor showed excellent selectivity, good stability and reversibility, and could detect the Gly in real samples rapidly and sensitively.

Schematic illustration of the experimental procedure to detect Gly using the MIPPy electrode

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Contact No. 31471654), the National Key Technology R&D Program for the 12th five-year plan (2014BAD13B05-05), the Agricultural Public Welfare Project and Innovative Research Team in Chinese academy of agricultural sciences (201203094), and the Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture of China (2016-KF-10).

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Correspondence to Yongxin She or Jing Wang.

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Zhang, C., She, Y., Li, T. et al. A highly selective electrochemical sensor based on molecularly imprinted polypyrrole-modified gold electrode for the determination of glyphosate in cucumber and tap water. Anal Bioanal Chem 409, 7133–7144 (2017). https://doi.org/10.1007/s00216-017-0671-5

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Keywords

  • Electrochemical sensor
  • Molecularly imprinted polypyrrole
  • Glyphosate
  • Cucumber
  • Tap water