Analytical and Bioanalytical Chemistry

, Volume 409, Issue 27, pp 6305–6314 | Cite as

Plasmonic cell nanocoating: a new concept for rapid microbial screening

  • Ke Xu
  • Minh-Phuong N. Bui
  • Aiqin Fang
  • Abdennour Abbas
Paper in Forefront

Abstract

Nanocoating of single microbial cells with gold nanostructures can confer optical, electrical, thermal, and mechanical properties to microorganisms, thus enabling new avenues for their control, study, application, and detection. Cell nanocoating is often performed using layer-by-layer (LbL) deposition. LbL is time-consuming and relies on nonspecific electrostatic interactions, which limit potential applications for microbial diagnostics. Here, we show that, by taking advantage of surface molecules densely present in the microbial outer layers, cell nanocoating with gold nanoparticles can be achieved within seconds using surface molecules, including disulfide- bond-containing (Dsbc) proteins and chitin. A simple activation of these markers and their subsequent interaction with gold nanoparticles allow specific microbial screening and quantification of bacteria and fungi within 5 and 30 min, respectively. The use of plasmonics and fluorescence as transduction methods offers a limit of detection below 35 cfu mL–1 for E. coli bacteria and 1500 cfu mL–1 for M. circinelloides fungi using a hand-held fluorescent reader.

Graphical abstract

A new concept for rapid microbial screening by targeting disulfide - bond-containing (Dsbc) proteins and chitin with reducing agents and gold nanoparticles.

Keywords

Cell nanocoating Gold nanoparticles Disulfide-bond containing (Dsbc) surface proteins Chitin Microbial screening 

Notes

Acknowledgments

The authors thank Dr. Renu Singh for help acquiring the Raman spectroscopy data and Yan Wu for help with microbial culture. The authors are grateful for the financial support of the National Science Foundation award No. 1605191, the University of Minnesota MnDRIVE Global Food Venture, the USDA National Institute of Food and Agriculture, Hatch project 1006789, General Mills, Schwan Food Company Graduate Fellowship, and the Midwest Dairy Association.

Compliance with ethical standards

This study has been approved by the Ethical committee of University of Minnesota and has been performed in accordance to the ethical standards and guidelines of University of Minnesota.

Conflict of interest

Dr. Abbas has filled out an international patent application no. PCT/US2017/015044, related to “Methods of attaching probes to microorganisms and methods of use”. The authors declare that they have no financial or nonfinancial conflict of interest.

Supplementary material

216_2017_612_MOESM1_ESM.pdf (1.3 mb)
ESM 1 (PDF 1343 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of Bioproducts and Biosystems EngineeringUniversity of Minnesota Twin CitiesSaint PaulUSA

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