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Analytical and Bioanalytical Chemistry

, Volume 409, Issue 27, pp 6405–6414 | Cite as

Biofluid pretreatment using gradient insulator-based dielectrophoresis: separating cells from biomarkers

Research Paper

Abstract

Blood is one of the most important biofluids used for clinical diagnostics. Cells and proteins in the blood can provide a rich source of information for the evaluation of human health. Efficient separation of blood components is a necessary process in order to minimize the interference of unwanted components during sensing, separation, and detection. In this paper, an insulator-based gradient dielectrophoretic device has been applied to separate red blood cells from model protein biomarkers for myocardial infarction in buffer. Within one min, red blood cells are largely depleted regardless of the minimum adherence on the channel wall. Considering the adhered red blood cells will not be transported further, a purified protein solution can be delivered for potential downstream processing or detection.

Graphical Abstract

Keywords

Red blood cell Protein biomarker Dielectrophoresis Separation Purification Myocardial infarction 

Abbreviations

DEP

Dielectrophoresis

EK

Electrokinesis

EO

Electroosmosis

EP

Electrophoresis

FI

Fluorescence intensity

g-iDEP

Gradient insulator-based dielectrophoresis

H-FABP

Human heart-type fatty acid binding protein

MI

Myocardial infarction

MyO

Human myoglobin

RBC

Red blood cell

ROI

Region of interest

Notes

Acknowledgements

This work was supported, in part, by the National Institutes of Health grants R21EB010191-02, 1R03AI094193-01, 1R03AI099740-01, and R03AI111361-01.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest. The study has been approved by ASU Institutional Biosafety Committee and all the experimental procedures were performed in accordance with its ethical standards. There was informed consent for all blood sample used.

Supplementary material

216_2017_582_MOESM1_ESM.pdf (161 kb)
ESM 1 (PDF 161 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.School of Molecular SciencesArizona State UniversityTempeUSA

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