Analytical and Bioanalytical Chemistry

, Volume 409, Issue 27, pp 6387–6396 | Cite as

Evaluation of blood adsorption onto dialysis membranes by time-of-flight secondary ion mass spectrometry and near-field infrared microscopy

  • Satoka Aoyagi
  • Kiyoshi Abe
  • Takayuki Yamagishi
  • Hideo Iwai
  • Satoru Yamaguchi
  • Takashi Sunohara
Research Paper


Blood adsorption onto the inside surface of hollow fiber dialysis membranes was investigated by means of time-of-flight secondary ion mass spectrometry (TOF-SIMS) and near-field infrared microscopy (NFIR) in order to evaluate the biocompatibility and permeability of dialysis membranes. TOF-SIMS is useful for the imaging of particular molecules with a high spatial resolution of approximately 100 nm. In contrast, infrared spectra provide quantitative information and NFIR enables analysis with a high spatial resolution of less than 1 μm, which is close to the resolution of TOF-SIMS. A comparison was made of one of the most widely used dialysis membranes made of polysulfone (PSf), that has an asymmetric and inhomogeneous pore structure, and a newly developed asymmetric cellulose triacetate (ATA) membrane that also has an asymmetric pore structure, even though the conventional cellulose triacetate membrane has a symmetric and homogeneous pore structure. As a result, it was demonstrated that blood adsorption on the inside surface of the ATA membrane is more reduced than that on the PSf membrane.

Graphical abstract

Analysis of blood adsorption on inside surface of hollow fiber membrane


Time-of-flight secondary ion mass spectrometry (TOF-SIMS) Near-field infrared microscopy (NFIR) Dialysis membrane Blood adsorption 



The authors thank Dr. Shinichi Iida, Mr. Takuya Miyayama, and Mr. Takahiro Suzuki from Ulvac-Phi for their support on TOF-SIMS measurement.

Compliance with ethical standards

Conflict of interest

Satoka Aoyagi has received research grants from Nipro Corporation and this study was partly funded by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP17K05908. Hideo Iwai and this study were partly funded by National Institute for Materials Science (NIMS) microstructural characterization platform as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

The other co-authors declare that they have no conflict of interest.

Ethics approval and consent to participate

All procedures performed in studies involving human participants were in accordance with the ethical standards of the ethical committee of Seikei University and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent was obtained from all individual participants included in the study.

Supplementary material

216_2017_578_MOESM1_ESM.pdf (9.6 mb)
ESM 1 (PDF 9802 kb).


  1. 1.
    Shang M, Matsuyama H, Maki T, Teramoto M, Lioyd DR. Effect of crystallization and liquid–liquid phase separation on phase-separation kinetics in poly(ethylene-co-vinyl alcohol)/glycerol solution. J Polym Sci B Polym Phys. 2003;41:194–201.CrossRefGoogle Scholar
  2. 2.
    Mangindaan D, Yared I, Kurniawan H, Sheu JR, Wang MJ. Modulation of biocompatibility on poly(vinylidene fluoride) and polysulfone by oxygen plasma treatment and dopamine coating. J Biomed Mater Res A. 2012;100(11):3177–88.CrossRefGoogle Scholar
  3. 3.
    Hayama M, Yamamoto K, Kohori F, Uesaka T, Ueno Y, Sugaya H, et al. Nanoscopic behavior of polyvinylpyrrolidone particle on polysulfone/polyvinylpyrrolidone film. Biomaterials. 2004;25:1019–28.CrossRefGoogle Scholar
  4. 4.
    Kuragano T, Kuno T, Takahashi Y, Yamamoto C, Nagura Y, Takahashi S, et al. Comparison of the effects of cellulose triacetate and polysulfone membrane on GPIIb/IIIa and platelet activation. Blood Purif. 2003;21:176–82.CrossRefGoogle Scholar
  5. 5.
    Mineshima M, Ishimori I, Ishida K, Hoshino T, Kaneko I, Sato Y, et al. Effects of internal filtration on the solute removal efficiency of a dialyzer. ASAIO J. 2000;46:456–60.CrossRefGoogle Scholar
  6. 6.
    Sunohara T, Masuda T. Cellulose triacetate as a high-performance membrane. In: Saito A, Kawanishi H, Yamashita AC, Mineshima M, editors. High-performance membrane dialyzers. Contrib Nephrol vol. 173. Basel: Karger; 2011. p. 156–63.CrossRefGoogle Scholar
  7. 7.
    Sunohara T, Masuda T. Fundamental characteristics of the newly developed ATATM membrane dialyzer. In: Kawanishi H, Takemoto Y, editors. Scientific aspects of dialysis therapy: JSDT/ISBP anniversary edition. Contrib Nephrol. Basel: Karger; 2017;189:215–221. doi: 10.1159/000451044).
  8. 8.
    Marques B, Pinheiro KF, Carmo LP, Costa MC, Abensure H. Anaphylactic reaction induced by a polysulfone/ polyvinylpyrrolidone membrane in the 10th session of hemodialysis with the same dialyzer. Hemodial Int. 2011;15:399–403.CrossRefGoogle Scholar
  9. 9.
    Rafael JSV, Gonzalez E, Quirce S, Diaz R, Alvarez L, Menendez D, et al. Hypersensitivity reactions to synthetic haemodialysis membranes. Nefrologia. 2014;34:520–5.Google Scholar
  10. 10.
    Olafiranye F, Kyaw W, Olafiranye O. Resolution of dialyzer membrane-associated thrombocytopenia with use of cellulose triacetate membrane: a case report. Case Rep Med. 2011;2011:134295.CrossRefGoogle Scholar
  11. 11.
    Kiaii M, Djurdjev O, Farah M, Levin A, Jung B, MacRae J. Use of electron-beam sterilized hemodialysis membranes and risk of thrombocytopenia. J Am Med Assoc. 2011;306:1679–87.CrossRefGoogle Scholar
  12. 12.
    Vickerman JC. Molecular imaging and depth profiling by mass spectrometry—SIMS, MALDI or DESI? Analyst. 2001;136:2199–217.CrossRefGoogle Scholar
  13. 13.
    Gilmore IS. SIMS of organics—advances in 2D and 3D imaging and future outlook. J Vac Sci Technol A. 2013;31:050819.CrossRefGoogle Scholar
  14. 14.
    Aoyagi S, Hayama M, Hasegawa U, Sakai K, Tozu M, Hoshi T, et al. Estimation of protein adsorption on dialysis membrane by means of TOF-SIMS imaging. J Membr Sci. 2004;236:91–9.CrossRefGoogle Scholar
  15. 15.
    Aoyagi S, Takesawa A, Yamashita AC, Kudo M. Mutual information theory for biomedical applications. Appl Surf Sci. 2006;252:6697–701.CrossRefGoogle Scholar
  16. 16.
    Aoyagi S, Shimanouchi T, Kawashima T, Iwai H. TOF-SIMS observation for evaluating the interaction between amyloid β and lipid membranes. Anal Bioanal Chem. 2015;407:2859–63.CrossRefGoogle Scholar
  17. 17.
    Koga S, Yakushiji T, Matsuda M, Yamamoto K, Sakai K. Functional-group analysis of polyvinylpyrrolidone on the inner surface of hollow-fiber dialysis membranes, by near-field infrared microspectroscopy. J Membr Sci. 2010;355:208–13.CrossRefGoogle Scholar
  18. 18.
    Yamagishi T, Honobe K, Aoyagi S, Okawa M, Kawashima T. Multivariate analysis applied to polymer imaging data obtained by near-field infrared microscopy. e-J Surf Sci Nanotechnol. 2017;15:19–24.CrossRefGoogle Scholar
  19. 19.
    Lee JLS, Gilmore IS. The application of multivariate data analysis techniques in surface analysis. In: Vickerman JC, Gilmore IS, editors. Surface analysis—the principal techniques. 2nd ed. New York: Wiley; 2009. p. 563–612.CrossRefGoogle Scholar
  20. 20.
    Lee JLS, Gilmore IS, Fletcher IW, Seah MP. Multivariate image analysis strategies for ToF-SIMS images with topography. Surf Interface Anal. 2009;41:653–65.CrossRefGoogle Scholar
  21. 21.
    Lee JLS, Gilmore IS, Seah MP. Quantification and methodology issues in multivariate analysis of ToF-SIMS data for mixed organic systems. Surf Interface Anal. 2008;40:1–14.CrossRefGoogle Scholar
  22. 22.
    de Kronig R. On the theory of dispersion of X-rays. J Opt Soc Am. 1926;12:547–57.CrossRefGoogle Scholar
  23. 23.
    Börner K, Malmberg P, Månsson JE, Nygren H. Molecular imaging of lipids in cells and tissues. Int J Mass Spectrom. 2007;260:128–36.CrossRefGoogle Scholar
  24. 24.
    Mantus DS, Ratner BD, Carlson BA, Moulder JF. Static secondary ion mass spectrometry of adsorbed proteins. Anal Chem. 1993;65:1431–8.CrossRefGoogle Scholar
  25. 25.
    Lhoest JB, Detrait E, van den Bosch de Aguilar P, Bertrand P. Fibronectin adsorption, conformation, and orientation on polystyrene substrates studied by radiolabeling, XPS, and ToF SIMS. J Biomed Mater Res. 1998;41:95–103.CrossRefGoogle Scholar
  26. 26.
    Wagner MS, Castner DG. Characterization of adsorbed protein films by time-of-flight secondary ion mass spectrometry with principal component analysis. Langmuir. 2001;17:4649–60.CrossRefGoogle Scholar
  27. 27.
    Mushtaq A, Mukhtar HB, Shariff AM. FTIR study of enhanced polymeric blend membrane with amines. Res J Appl Sci Eng Technol. 2014;7:1811–20.Google Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Satoka Aoyagi
    • 1
  • Kiyoshi Abe
    • 1
  • Takayuki Yamagishi
    • 1
  • Hideo Iwai
    • 2
  • Satoru Yamaguchi
    • 3
  • Takashi Sunohara
    • 3
  1. 1.Materials and Life SciSeikei UniversityTokyoJapan
  2. 2.Materials Analysis StationNational Institute for Materials ScienceIbarakiJapan
  3. 3.Nipro CorporationOsakaJapan

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