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Rapid electrochemical quantification of Salmonella Pullorum and Salmonella Gallinarum based on glucose oxidase and antibody-modified silica nanoparticles

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Abstract

In this article, a facile and sensitive electrochemical method for quantification of Salmonella Pullorum and Salmonella Gallinarum (S. Pullorum and S. Gallinarum) was established by monitoring glucose consumption with a personal glucose meter (PGM). Antibody-functionalized magnetic nanoparticles (IgG-MNPs) were used to capture and enrich S. Pullorum and S. Gallinarum, and IgG-MNPs-S. Pullorum and IgG-MNPs-S. Gallinarum complexes were magnetically separated from a sample using a permanent magnet. The trace tag was prepared by loading polyclonal antibodies and high-content glucose oxidase on amino-functionalized silica nanoparticles (IgG-SiNPs-GOx). With a sandwich-type immunoassay format, IgG-SiNPs-GOx were added into the above mixture solution and conjugated to the complexes, forming sandwich composites IgG-MNPs/S. Pullorum and S. Gallinarum/IgG-SiNPs-GOx. The above sandwich composites were dispersed in glucose solution. Before and after the hydrolysis of glucose, the concentration of glucose was measured using PGM. Under optimal conditions, a linear relationship between the decrease of glucose concentration and the logarithm of S. Pullorum and S. Gallinarum concentration was obtained in the concentration range from 1.27 × 102 to 1.27 × 105 CFU mL−1, with a detection limit of 7.2 × 101 CFU mL−1 (S/N = 3). This study provides a portable, low-cost, and quantitative analytical method for bacteria detection; thus, it has a great potential in the prevention of disease caused by S. Pullorum and S. Gallinarum in poultry.

A schematic illustration of the fabrication process of IgG-SiNPs-GOD nanomaterials (A) and IgG-MNPs (B) and experimental procedure of detection of S. Pullorum and S. Gallinarum using GOD-functionalized silica nanospheres as trace tags based on PGM (C).

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Acknowledgements

This work was supported by a grant from the National Natural Science Foundation of Zhejiang Province (LY17C2000003), the Food Science and Engineering the most important discipline of Zhejiang Province (JYTSP20141062), the Zhejiang Public Innovation Platform Analysis and Testing Project (2015C37023), the Talent Training Provincial Superior Paper Funded Project (1110JY1412001P), the Technological Innovation Project of Zhejiang Gongshang University (CX201610024, CX201610019), the Graduate Research Innovation Fund Project of Zhejiang Gongshang University, and the Plans of College Students in Zhejiang Province and Technology Innovation Activities (Acrobatic Tender Grass Talent Programme) Project (1110JQ4212048G, 1110KZN0213112G).

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  1. Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xuezheng Street, Xiasha Higher Education Zone, Hangzhou, Zhejiang, 310018, China

    Yiheng Luo, Wenchao Dou & Guangying Zhao

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  1. Yiheng Luo
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  2. Wenchao Dou
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  3. Guangying Zhao
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Correspondence to Wenchao Dou or Guangying Zhao.

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Luo, Y., Dou, W. & Zhao, G. Rapid electrochemical quantification of Salmonella Pullorum and Salmonella Gallinarum based on glucose oxidase and antibody-modified silica nanoparticles. Anal Bioanal Chem 409, 4139–4147 (2017). https://doi.org/10.1007/s00216-017-0361-3

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  • DOI: https://doi.org/10.1007/s00216-017-0361-3

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