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Analytical strategy for determination of known and unknown destruxins using hybrid quadrupole-Orbitrap high-resolution mass spectrometry

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Abstract

An analytical strategy based on a hybrid quadrupole-Orbitrap mass spectrometry was proposed for the simultaneous screening of known destruxins and characterization of potential members of this class of secondary metabolites, in order to evaluate the metabolite production of entomopathogenic fungi used as biocontrol agents. Initially, the fragmentation pathway of the known and commercially available destruxin A was established combining high resolution mass spectrometry (HRMS) and multiple stage MS data in order to obtain the strategy for the characterization of other destruxins for which reference standards were not available. Nineteen known destruxins including A, B, C, D, Ed, F, A1, B1, Ed1, A2, B2, D2, A3, DesmA, DesmB, DesmC, DesmB2, and two chloro-derivatives (Cl and E2 chlorohydrin) were unequivocally identified in Metarhizium brunneum using the proposed strategy. In addition, four unknown destruxins, namely C1, Ed2, G, and G1, were structurally elucidated and characterized for the first time in this fungal strain.

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Acknowledgements

Natalia Arroyo-Manzanares received a post-doctoral grant from the University of Granada. This research has been partially supported by the European Community’s Seventh Framework Program Grant (FP7-ENV.2011.3.1.9-1 ECO-INNOVATION, INBIOSOIL, Grant Agreement no. 282767).

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Author notes

  1. Natalia Arroyo-Manzanares and José Diana Di Mavungu contributed equally to this work.

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium

    Natalia Arroyo-Manzanares, José Diana Di Mavungu & Sarah De Saeger

  2. Department of Analytical Chemistry, University of Cordoba, Annex C3 Building, Campus of Rabanales, 14071, Cordoba, Spain

    Natalia Arroyo-Manzanares & Lourdes Arce

  3. Department of Agricultural and Forestry Sciences, ETSIAM, University of Cordoba, Building C4, Campus of Rabanales, 14071, Cordoba, Spain

    Inmaculada Garrido-Jurado & Enrique Quesada-Moraga

  4. Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium

    Lynn Vanhaecke

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  1. Natalia Arroyo-Manzanares
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  2. José Diana Di Mavungu
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  3. Inmaculada Garrido-Jurado
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  4. Lourdes Arce
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  5. Lynn Vanhaecke
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  6. Enrique Quesada-Moraga
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Correspondence to Natalia Arroyo-Manzanares or José Diana Di Mavungu.

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Arroyo-Manzanares, N., Diana Di Mavungu, J., Garrido-Jurado, I. et al. Analytical strategy for determination of known and unknown destruxins using hybrid quadrupole-Orbitrap high-resolution mass spectrometry. Anal Bioanal Chem 409, 3347–3357 (2017). https://doi.org/10.1007/s00216-017-0276-z

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  • DOI: https://doi.org/10.1007/s00216-017-0276-z

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