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Vortex-assisted surfactant-enhanced emulsification microextraction combined with LC–MS/MS for the determination of glucocorticoids in water with the aid of experimental design

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Abstract

An efficient and inexpensive method using vortex-assisted surfactant-enhanced emulsification microextraction (VASEME) based on solidification of floating organic droplet coupled with ultraperformance liquid chromatography–tandem mass spectrometry is proposed for the analysis of glucocorticoids in water samples (river water and hospital wastewater). VASEME was optimized by the experimental validation of Plackett–Burman design and central composite design, which has been co-related to experimental design. Plackett–Burman design showed that factors such as vortex time, surfactant concentration, and pH significantly affect the extraction efficiency of the method. Method validation was characterized by an acceptable calibration range of 1–1000 ng L−1, and the limit of detection was in the range from 2.20 to 8.12 ng L−1 for glucocorticoids. The proposed method was applied to determine glucocorticoids in river water and hospital wastewater in Lucknow, India. It is reliable and rapid and has potential application for analysis of glucocorticoids in environmental aqueous samples.

Low density based extraction of gluococorticoids by using design of experiment

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Acknowledgements

The authors express their gratitude to the Director, CSIR-Indian Institute of Toxicology Research, Lucknow, India, for providing the necessary facilities for this research study. The authors also thank the University Grant Commission, GAP project-155, New Delhi, India, and the CSIR-INDEPTH project for financial assistance. We are also thankful to B.D. Bhattacharji for support in terms of manuscript preparation.

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    1. Academy of Scientific & Innovative Research (AcSIR), CSIR-IITR Campus, Lucknow, 226001, India

      Ankita Asati & Devendra K. Patel

    2. Analytical Chemistry Laboratory, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan 31, Mahatma Gandhi Marg, PO Box 80, Lucknow, 226001, Uttar Pradesh, India

      Ankita Asati, G. N. V. Satyanarayana & Devendra K. Patel

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    1. Ankita Asati
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    Correspondence to Devendra K. Patel.

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    This is CSIR-IITR institutional communication no. 3448.

    Ankita Asati and G. N. V. Satyanarayana contributed equally to this work.

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    Asati, A., Satyanarayana, G.N.V. & Patel, D.K. Vortex-assisted surfactant-enhanced emulsification microextraction combined with LC–MS/MS for the determination of glucocorticoids in water with the aid of experimental design. Anal Bioanal Chem 409, 2905–2918 (2017). https://doi.org/10.1007/s00216-017-0236-7

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