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Identification and quantification of nitrofurazone metabolites by ultraperformance liquid chromatography–quadrupole time-of-flight high-resolution mass spectrometry with precolumn derivatization

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Abstract

An ultraperformance liquid chromatography–quadrupole time-of-flight high-resolution mass spectrometry method was developed and validated for the determination of nitrofurazone metabolites. Precolumn derivatization with 2,4-dinitrophenylhydrazine and p-dimethylaminobenzaldehyde as an internal standard was used successfully to determine the biomarker 5-nitro-2-furaldehyde. In negative electrospray ionization mode, the precise molecular weights of the derivatives were 320.0372 for the biomarker and 328.1060 for the internal standard (relative error 1.08 ppm). The matrix effect was evaluated and the analytical characteristics of the method and derivatization reaction conditions were validated. For comparison purposes, spiked samples were tested by both internal and external standard methods. The results show high precision can be obtained with p-dimethylaminobenzaldehyde as an internal standard for the identification and quantification of nitrofurazone metabolites in complex biological samples.

A simplified preparation strategy for biological samples

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Acknowledgements

This research was supported by Zhejiang Provincial Natural Science Foundation of China (LY17C200009). The authors thank the Scientific Research Foundation (SRF, Q1435) and Zhejiang Ocean University and Science and Technology Plan of Zhejiang province (2016 F30022) for supporting the research.

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Authors and Affiliations

  1. Key Lab of Mariculture & Enhancement, Marine Fisheries Research Institute of Zhejiang Province, Zhoushan, 316000, China

    Shuai Zhang, PeiPei Li, Zhongyong Yan, Ju Long & Xiaojun Zhang

  2. Marine and Fisheries Research Institute, Zhejiang Ocean University, Zhejiang, Zhoushan, 316000, China

    Shuai Zhang

Authors
  1. Shuai Zhang
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  2. PeiPei Li
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  3. Zhongyong Yan
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  4. Ju Long
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  5. Xiaojun Zhang
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Corresponding author

Correspondence to Xiaojun Zhang.

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Experimental protocols complied with the Guide for the Care and Use of Laboratory Animals of the China Association for Ethical Studies and were approved by the Institutional Animal Care and Use Committee of Zhejiang Ocean University.

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The authors declare that they have no conflict of interest.

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Zhang, S., Li, P., Yan, Z. et al. Identification and quantification of nitrofurazone metabolites by ultraperformance liquid chromatography–quadrupole time-of-flight high-resolution mass spectrometry with precolumn derivatization. Anal Bioanal Chem 409, 2255–2260 (2017). https://doi.org/10.1007/s00216-017-0191-3

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