Analytical and Bioanalytical Chemistry

, Volume 408, Issue 27, pp 7775–7783 | Cite as

An integrated lab-on-a-chip-based electrochemical biosensor for rapid and sensitive detection of cancer biomarkers

  • Yildiz Uludag
  • Fehmi Narter
  • Erkin Sağlam
  • Güzin Köktürk
  • M. Yağmur Gök
  • Mete Akgün
  • Serkan Barut
  • Sinan Budak
Research Paper


Recent advances in the area of biosensor technology and microfluidic applications have enabled the miniaturisation of the sensing platforms. Here we describe a new integrated and fully automated lab-on-a-chip-based biosensor device prototype (MiSens) that has potential to be used for point-of-care cancer biomarker testing. The key features of the device include a new biochip, a device integrated microfluidic system and real-time amperometric measurements during the flow of enzyme substrate. For ease of use, a new plug and play type sensor chip docking station has been designed. This system allows the formation of an ∼7 μL capacity flow cell on the electrode array with the necessary microfluidic and electronic connections with one move of a handle. As a case study, the developed prototype has been utilised for the detection of prostate-specific antigen (PSA) level in serum that is routinely used as a biomarker for the diagnosis of prostate cancer. The patient samples from a nearby hospital have been collected and tested using the MiSens device, and the results have been compared to the hospital results. The obtained results indicate the potential of the MiSens device as a useful tool for point-of-care testing.

Graphical abstract

Microfluidics integrated and automated electrochemical biosensor device “MiSens” has been designed and fabricated by a multidisciplinary team and utilised to detect PSA from clinical samples.


Integrated microfluidics Real-time electrochemical profiling Amperometry MiSens Biosensor Cancer biomarker 



The project is supported by BILGEM-TUBITAK (The Scientific and Technological Research Council of Turkey) (grant no: S569000 and 100121) and the Republic of Turkey Ministry of Development Infrastructure Grant (no: 2011K120020). We gratefully acknowledge Aylin Ersoy, Atike Demiralp, Hakkı Aktepe, Tugba Yurt and Muammer Karadağ from BILGEM-TUBITAK for their contribution to the fabrication of the electrode arrays and the device. We gratefully acknowledge Dr. Hasan Güner and Dr. Erol Özgür from Bilkent University for their help with XPS measurements.

Compliance with ethical standards

Conflict of interest

On behalf of myself and other co-authors, I state that there is no conflict of interest in this paper.

Ethical approval

Patient samples were collected from Kartal Lütfi Kırdar Education and Research Hospital (İstanbul, Turkey). The ethical approval was obtained from the same hospital’s Research Ethics Committee. All the participants gave their informed consent before sample collection.

Supplementary material

216_2016_9879_MOESM1_ESM.pdf (1.3 mb)
ESM 1 (PDF 1310 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Yildiz Uludag
    • 1
  • Fehmi Narter
    • 2
  • Erkin Sağlam
    • 2
  • Güzin Köktürk
    • 1
  • M. Yağmur Gök
    • 1
  • Mete Akgün
    • 1
  • Serkan Barut
    • 1
  • Sinan Budak
    • 1
  1. 1.Bioelectronic Devices and Systems Group, UEKAE-BILGEM-The Scientific and Technological Research Council of Turkey (TUBITAK)GebzeTurkey
  2. 2.Kartal Dr .Lütfi Kırdar Education and Research Hospital Urology ClinicKartalTurkey

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