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Photosensitive diazotized poly(ethylene glycol) covalent capillary coatings for analysis of proteins by capillary electrophoresis

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Abstract

A new method for the fabrication of covalently cross-linked capillary coatings of poly(ethylene glycol) (PEG) is described using diazotized PEG (diazo-PEG) as a new photosensitive coating agent. The film of diazo-PEG depends on ionic bonding and was first prepared on the inner surface of capillary by self-assembly, and ionic bonding was converted into covalent bonding after reaction of ultraviolet light with diazo groups through unique photochemical reaction. The covalently bonded coating impedance adsorption of protein on the central surface of capillary and hence the four proteins ribonuclease A, cytochrome c, bovine serum albumin, and lysosome can be baseline separated by using capillary electrophoresis (CE). The covalently cross-linked diazo-PEG capillary column coatings not only improved the CE separation performance for proteins compared to non-covalently cross-linked coatings or bare capillary but also showed a remarkable chemical solidity and repeatability. Because photosensitive diazo-PEG took the place of the highly noxious and silane moisture-sensitive coating reagents in the fabrication of covalent coating, this technique shows the advantage of being environment-friendly and having a high efficiency for CE to make the covalently bonded capillaries.

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Acknowledgments

This work is financially supported by the Natural Science Foundation of China (21375069, 21404065, 21574072), the Natural Science Foundation for Distinguished Young Scientists of Shandong Province (JQ201403), the Project of Shandong Province Higher Educational Science and Technology Program (J15LC20), the Graduate Education Innovation Project of Shandong Province (SDYY14028), the Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry (20111568), and the Postdoctoral Scientific Research Foundation of Qingdao (2015128).

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Authors and Affiliations

  1. College of Materials Science and Engineering, Qingdao University, Qingdao, Shandong, 266071, China

    Bing Yu, Xin Chen, Hailin Cong, Xi Shu & Qiaohong Peng

  2. Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, Qingdao, Shandong, 266071, China

    Bing Yu & Hailin Cong

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  1. Bing Yu
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  2. Xin Chen
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  3. Hailin Cong
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  4. Xi Shu
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Correspondence to Bing Yu or Hailin Cong.

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Yu, B., Chen, X., Cong, H. et al. Photosensitive diazotized poly(ethylene glycol) covalent capillary coatings for analysis of proteins by capillary electrophoresis. Anal Bioanal Chem 408, 6781–6788 (2016). https://doi.org/10.1007/s00216-016-9804-5

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  • DOI: https://doi.org/10.1007/s00216-016-9804-5

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