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Differentiating Pseudomonas sp. strain ADP cells in suspensions and biofilms using Raman spectroscopy and scanning electron microscopy

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Abstract

High quality spectra of Pseudomonas sp. strain ADP in the planktonic and biofilm state were obtained using Raman microspectroscopy. These spectra enabled the identification of key differences between free and biofilm cells in the fingerprint region of Raman spectra in the nucleic acid, carbohydrate, and protein regions. Scanning electron microscopy (SEM) enabled detailed visualization of ADP biofilm with confirmation of associated extracellular matrix structure. Following extraction and Raman analysis of extracellular polymeric substances, Raman spectral differences between free and biofilm cells were largely attributed to the contribution of extracellular matrix components produced in mature biofilms. Raman spectroscopy complemented with SEM proves to be useful in distinguishing physiological properties among cells of the same species.

Raman spectroscopy complemented with SEM proves to be useful in distinguishing physiological properties among cells of the same species

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Acknowledgments

This work was supported by the Graduate Assistance in Areas of National Need (P200A090350) and the University of Iowa Center for Health Effects of Environmental Contamination. We thank the University of Iowa Central Microscopy Research Facility for access to the Raman confocal microscope and scanning electron microscope.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, University of Iowa, 4133 Seamans Center, Iowa City, IA, 52242, USA

    Victoria A. Henry, Julie L. P. Jessop & Tonya L. Peeples

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  1. Victoria A. Henry
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  2. Julie L. P. Jessop
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  3. Tonya L. Peeples
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Correspondence to Tonya L. Peeples.

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Henry, V.A., Jessop, J.L.P. & Peeples, T.L. Differentiating Pseudomonas sp. strain ADP cells in suspensions and biofilms using Raman spectroscopy and scanning electron microscopy. Anal Bioanal Chem 409, 1441–1449 (2017). https://doi.org/10.1007/s00216-016-0077-9

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  • DOI: https://doi.org/10.1007/s00216-016-0077-9

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