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Exploiting NanoLuc luciferase for smartphone-based bioluminescence cell biosensor for (anti)-inflammatory activity and toxicity

Abstract

The availability of smartphones with high-performance digital image sensors and processing power has completely reshaped the landscape of point-of-need analysis. Thanks to the high maturity level of reporter gene technology and the availability of several bioluminescent proteins with improved features, we were able to develop a bioluminescence smartphone-based biosensing platform exploiting the highly sensitive NanoLuc luciferase as reporter. A 3D-printed smartphone-integrated cell biosensor based on genetically engineered Hek293T cells was developed. Quantitative assessment of (anti)-inflammatory activity and toxicity of liquid samples was performed with a simple and rapid add-and-measure procedure. White grape pomace extracts, known to contain several bioactive compounds, were analyzed, confirming the suitability of the smartphone biosensing platform for analysis of untreated complex biological matrices. Such approach could meet the needs of small medium enterprises lacking fully equipped laboratories for first-level safety tests and rapid screening of new bioactive products.

Smartphone-based bioluminescence cell biosensor

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Acknowledgements

The grape pomace samples were obtained in the frame of a project supported by the Sadam Engineering (Sadam Eridania Spa group, Bologna, Italy) to Annalisa Tassoni. This research was sponsored in part by the NATO Science for Peace and Security Programme under Grant No. 985042.

Author information

Correspondence to Aldo Roda or Elisa Michelini.

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Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Published in the topical collection Highlights of Analytical Chemical Luminescence with guest editors Aldo Roda, Hua Cui, and Chao Lu.

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Cevenini, L., Calabretta, M.M., Lopreside, A. et al. Exploiting NanoLuc luciferase for smartphone-based bioluminescence cell biosensor for (anti)-inflammatory activity and toxicity. Anal Bioanal Chem 408, 8859–8868 (2016). https://doi.org/10.1007/s00216-016-0062-3

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Keywords

  • Bioluminescence
  • Smartphone
  • Anti-inflammatory activity
  • Toxicity
  • Portable analytical device
  • Cell biosensor