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Fig. 1a–g | Analytical and Bioanalytical Chemistry

Fig. 1a–g

From: Immobilization and detection of platelet-derived extracellular vesicles on functionalized silicon substrate: cytometric and spectrometric approach

Fig. 1a–g

Optical images of platelets and platelet-derived microvesicles (PMVs). Platelets obtained from PRP (platelet-rich plasma) were placed on siliconized (a) and collagen-coated (b, c) glass slides. Phalloidin staining revealed specific actin cytoskeleton organization. Thrombin activation produced more phalloidin-stained spots (c). Arrows indicate actin streaks from activated platelets, which correspond to actin-rich PMVs. ImageStreamX Mark II cytometry showed that most of the PMVs were gated between 0.13 and 0.22 μm in sidescatter (SSC) and bright-field channels (d). A minority (∼30 %) of the PMVs were positive for annexin V (e); most of the PMVs were double-positive for the antigens CD9 (exosome-specific) and CD61 (platelet-specific) (f). Selected images of microvesicles are shown in g. AF Alexa Fluor, PE phycoerythrin

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