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High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry

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Abstract

In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve about 125,880 cells per minute (more than one hundred and twenty five thousand cells per minute), even while employing cost-effective low frame rate cameras (120 fps). The throughput achieved here is a notable progression in the field of diagnostics as it enables rapid quantitative testing and analysis. We demonstrate the applicability of the instrument to point-of-care diagnostics, by performing blood cell counting. We report a comparative analysis between the counts (in cells per μl) obtained from our instrument, with that of a commercially available hematology analyzer.

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Acknowledgments

The authors thank Mr. Abhishek Pathak for preparing blood samples for these experiments. The authors acknowledge funding from Biotechnology Ignition Grant (BIG) of BIRAC. BIG team also acknowledges the support of incubation center, Robert-Bosch Center for Cyber-Physical Systems (RBCCPS), IISc.

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Authors and Affiliations

  1. Optics, Microfluidics Instrumentation Lab, Department of Instrumentation & Applied Physics, Indian Institute of Science (IISc), Bangalore, India, 560012

    Veerendra Kalyan Jagannadh & Sai Siva Gorthi

  2. Robert Bosch Centre For Cyber-Physical Systems, Indian Institute of Science (IISc), Bangalore, India, 560012

    Bindu Prabhath Bhat, Lourdes Albina Nirupa Julius & Sai Siva Gorthi

Authors
  1. Veerendra Kalyan Jagannadh
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  2. Bindu Prabhath Bhat
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  3. Lourdes Albina Nirupa Julius
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  4. Sai Siva Gorthi
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Corresponding author

Correspondence to Sai Siva Gorthi.

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The authors declare no conflicts of Interest.

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Jagannadh, V.K., Bhat, B.P., Nirupa Julius, L.A. et al. High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry. Anal Bioanal Chem 408, 1909–1916 (2016). https://doi.org/10.1007/s00216-015-9301-2

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  • DOI: https://doi.org/10.1007/s00216-015-9301-2

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