Advertisement

Analytical and Bioanalytical Chemistry

, Volume 408, Issue 5, pp 1391–1397 | Cite as

A smart pipette for equipment-free separation and delivery of plasma for on-site whole blood analysis

  • Sung B. Im
  • Sang C. Kim
  • Joon S. ShimEmail author
Rapid Communication

Abstract

A novel device of smart pipette has been suggested to extract and deliver plasma from whole blood in a disposable format. By operating an on-chip disposable micropump, approximately 30 μL of plasma was obtained from 100 μL of whole blood within 5 min without any external equipment for point-of-care blood analysis.

Graphical Abstract

A novel device of smart pipette as a stand-alone lab on a chip (LOC) device has been successfully developed to rapidly extract and deliver 30 μL of plasma within 5 min from 100 μL of whole blood with 45 % hematocrit.

Keywords

Blood separation Micropump Lab on a chip Point-of-care testing Microfilter 

Notes

Acknowledgments

The authors gratefully acknowledge the support of this work by the Kwangwoon Research Grant of 2014. This research was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. NRF-2015R1D1A1A01060369) and Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2015 (Grants No. C0299553).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

216_2015_9259_MOESM1_ESM.pdf (709 kb)
ESM 1 (PDF 606 kb)
216_2015_9259_MOESM2_ESM.mp4 (18.9 mb)
ESM 2 (MP4 19360 kb)

References

  1. 1.
    Abe K, Sujuki K, Citterio D. Anal Chem. 2008;80:6928–34.CrossRefGoogle Scholar
  2. 2.
    Yang X, Forouzan O, Brown TP, Shevkoplyas SS. Lab Chip. 2012;12:274–80.CrossRefGoogle Scholar
  3. 3.
    Hou HW, Bhagat AAS, Lee WC, Huang S, Han J, Lim CT. Micromachines. 2011;2(3):319–43.CrossRefGoogle Scholar
  4. 4.
    Yager P, Domingo GJ, Gerdes J. Annu Rev Biomed Eng. 2008;10:107–44.CrossRefGoogle Scholar
  5. 5.
    Nivedita N, Papautsky I. Biomicrofluidics. 2013;7:054101.CrossRefGoogle Scholar
  6. 6.
    Lee MG, Choi SY, Kim HJ, Lim HK, Kim JH, Huh N, et al. Appl Phys Lett. 2011;98:253702.CrossRefGoogle Scholar
  7. 7.
    Son JH, Lee SH, Hong SW, Park SM, Lee J, Dickey AM, et al. Lap Chip. 2014;14:2287–92.CrossRefGoogle Scholar
  8. 8.
    Dimov IK, Basabe-Desmonts L, Garcia-Cordero JL, Ross BM, Ricco AJ, Lee LP. Lab Chip. 2011;11:845–50.CrossRefGoogle Scholar
  9. 9.
    Haeberle S, Brenner T, Zengerle R, Ducree J. Lab Chip. 2006;6:776–81.CrossRefGoogle Scholar
  10. 10.
    Lai S, Wang S, Luo J, Lee LJ, Yang ST, Madou MJ. Anal Chem. 2004;76:1832–7.CrossRefGoogle Scholar
  11. 11.
    Yang S, Undar A, Zahn JD. Lab Chip. 2006;6:871–80.CrossRefGoogle Scholar
  12. 12.
    Browne AW, Ramasamy L, Cripe TP, Ahn CH. Lap Chip. 2011;11:2440–6.CrossRefGoogle Scholar
  13. 13.
    Crowley TA, Pizziconi V. Lap Chip. 2005;5:922–9.CrossRefGoogle Scholar
  14. 14.
    Shim JS, Ahn CH. Lap Chip. 2012;12:863–6.CrossRefGoogle Scholar
  15. 15.
    Shim JS, Browne AW, Ahn CH. Biomed Microdevices. 2010;12:949–57.CrossRefGoogle Scholar
  16. 16.
    Thorslund S, Klett O, Nikolajeff F, Markides K. Bergquist. Biomed Microdevices. 2006;8:73–9.CrossRefGoogle Scholar
  17. 17.
    Au AK, Lai H, Utela BR, Folch A. Micromachines. 2011;2:179–220.CrossRefGoogle Scholar
  18. 18.
    Tracey MC, Johnston ID, Davis JB, Tan CKLJ. Micromech Microeng. 2006;16:1444–52.CrossRefGoogle Scholar
  19. 19.
    Li S, Chen S. Sensors Actuators A. 2003;104:151–61.CrossRefGoogle Scholar
  20. 20.
    Grover WH, Skelley AM, Liu CN, Lagally ET, Mathies RA. Sensors Actuators B. 2003;89:315–23.CrossRefGoogle Scholar
  21. 21.
    Unger MA, Chou HP, Thorsen T, Scherer A, Quake SR. Science. 2000;288:113–6.CrossRefGoogle Scholar
  22. 22.
    Iwai K, Sochol RD, Lee LP, Lin L. IEEE Int Conf Micro Electro Mech Syst Tech Dig, 25th. 2012;949–52.Google Scholar
  23. 23.
    Byun KI, Han ED, Kim BH, Seo YH. KMEMS Conf Dig, 12th. 2010;130–31.Google Scholar
  24. 24.
    Li CG, Lee K, Lee CY, Dangol M, Jung H. Adv Mater. 2012;24:4583–6.CrossRefGoogle Scholar
  25. 25.
    Juncker D, Schmid H, Drechsler U, Wolf H, Wolf M, Michel B, et al. Anal Chem. 2002;74:6139–44.CrossRefGoogle Scholar
  26. 26.
    Walker GM, Beebe DJ. Lab Chip. 2002;2:131–4.CrossRefGoogle Scholar
  27. 27.
    Hosokawa K, Sato K, Ichikawa N, Maeda M. Lab Chip. 2004;4:181–5.CrossRefGoogle Scholar
  28. 28.
    Browne AW, Rust MJ, Jung W, Lee SH, Ahn CH. Lap Chip. 2009;9:2941–6.CrossRefGoogle Scholar
  29. 29.
    Ahn CH, Choi JW, Beaucage G, Nevin JH, Lee JB, Puntambekar A, et al. Proc IEEE. 2004;92:154–73.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Bio IT Convergence Laboratory, Department of Electronics Convergence EngineeringKwangwoon UniversitySeoulRepublic of Korea

Personalised recommendations