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Fourier transform infrared spectroscopy (FTIR) characterization of the interaction of anti-cancer photosensitizers with dendrimers

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Abstract

The systemic or local administration of a photosensitizer for photodynamic therapy is highly limited by poor selectivity, rapid deactivation and long-lasting skin toxicity due to unfavorable biodistribution. Drug delivery systems based on nanocarriers may help specific and effective delivery of photosensitizers. In the present paper, the interaction of two photosensitizers, methylene blue and rose bengal, with phosphorous cationic and anionic dendrimers as potential nanocarriers, has been characterized. A novel method is presented based on the analysis of the infrared spectra of mixtures of photosensitizer and dendrimer. The capacity of dendrimers to bind the photosensitizers has been evaluated by obtaining the corresponding binding curves. It is shown that methylene blue interacts with both cationic and anionic dendrimers, whereas rose bengal only binds to the cationic ones. Dendrimers are shown to be potential nanocarriers for a specific delivery of both photosensitizers.

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Acknowledgments

This study was funded by the project “Phosphorus dendrimers as carriers of photosensitizers in photodynamic therapy and its combination with hyperthermia in in vitro studies” operated within the Foundation for Polish Science VENTURES Programme (Project VENTURES number VENTURES/2013-11/3) co-financed by the EU European Regional Development Fund and by the grant HARMONIA “Studying phosphorus dendrimers as systems transporting photosensitizers” no. UMO-2013/08/M/NZ1/00761 supported by National Science Centre.

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Authors and Affiliations

  1. Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland

    Monika Dabrzalska, Maria Bryszewska & Barbara Klajnert-Maculewicz

  2. ALBA Synchrotron, BP-1413 km 3,3, 08290, Cerdanyola del Vallès, Catalonia, Spain

    Nuria Benseny-Cases

  3. Biophysics Unit and Center of Studies in Biophysics, Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, 08193, Bellaterra, Catalonia, Spain

    Ramon Barnadas-Rodríguez & Josep Cladera

  4. PRES Sorbonne Paris Cité, CNRS UMR 860, Laboratoire de Chimie et de Biochimie pharmacologiques et toxicologique, Université Paris Descartes, 45 rue des Saints Pères, 75006, Paris, France

    Serge Mignani

  5. Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363, Lodz, Poland

    Maria Zablocka

  6. Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077, Toulouse, France

    Jean-Pierre Majoral

  7. Université de Toulouse, UPS, INPT, 31077, Toulouse Cedex 4, France

    Jean-Pierre Majoral

  8. Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, 01069, Dresden, Germany

    Barbara Klajnert-Maculewicz

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  1. Monika Dabrzalska
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  2. Nuria Benseny-Cases
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  4. Serge Mignani
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  5. Maria Zablocka
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  6. Jean-Pierre Majoral
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  7. Maria Bryszewska
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  8. Barbara Klajnert-Maculewicz
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  9. Josep Cladera
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Correspondence to Josep Cladera.

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Dabrzalska, M., Benseny-Cases, N., Barnadas-Rodríguez, R. et al. Fourier transform infrared spectroscopy (FTIR) characterization of the interaction of anti-cancer photosensitizers with dendrimers. Anal Bioanal Chem 408, 535–544 (2016). https://doi.org/10.1007/s00216-015-9125-0

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  • DOI: https://doi.org/10.1007/s00216-015-9125-0

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