Analytical and Bioanalytical Chemistry

, Volume 407, Issue 27, pp 8311–8320

Characterisation of a fibre optic Raman probe within a hypodermic needle

  • Ingeborg E. Iping Petterson
  • John C. C. Day
  • Leanne M. Fullwood
  • Benjamin Gardner
  • Nick Stone
Research Paper

DOI: 10.1007/s00216-015-9021-7

Cite this article as:
Iping Petterson, I.E., Day, J.C.C., Fullwood, L.M. et al. Anal Bioanal Chem (2015) 407: 8311. doi:10.1007/s00216-015-9021-7
Part of the following topical collections:
  1. Raman4Clinics

Abstract

We demonstrate the first use of a multifibre Raman probe that fits inside the bore of a hypodermic needle. A Raman probe containing multiple collection fibres provides improved signal collection efficiency in biological samples compared with a previous two-fibre design. Furthermore, probe performance (signal-to-noise ratios) compared favourably with the performance achieved in previous Raman microscope experiments able to distinguish between benign lymph nodes, primary malignancies in lymph nodes and secondary malignancies in lymph nodes. The experimental measurements presented here give an indication of the sampling volume of the Raman needle probe in lymphoid tissues. Liquid tissue phantoms were used that contained scattering medium encompassing a range of scattering properties similar to those of a variety of tissue types, including lymph node tissues. To validate the appropriateness of the phantoms, the sampling depth of the probe was also measured in excised lymph node tissue. More than 50 % of Raman photons collected were found to originate from between the tip of the needle and a depth of 500 μm into the tissue. The needle probe presented here achieves spectral quality comparable to that in numerous studies previously demonstrating Raman disease discrimination. It is expected that this approach could achieve targeted subcutaneous tissue measurements and be viable for use for the in vivo Raman diagnostics of solid organs located within a few centimetres below the skin’s surface.

Graphical Abstract

Schematic of multi-fibre Raman needle probe with disposible tips and proximal optical filtration

Keywords

Non-invasive Diagnostics Needle Raman spectroscopy Lymph nodes 

Supplementary material

216_2015_9021_MOESM1_ESM.pdf (72 kb)
ESM 1(PDF 71 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ingeborg E. Iping Petterson
    • 1
    • 2
  • John C. C. Day
    • 2
  • Leanne M. Fullwood
    • 1
    • 3
  • Benjamin Gardner
    • 1
  • Nick Stone
    • 1
    • 3
  1. 1.Biomedical Physics, School of Physics and AstronomyUniversity of ExeterExeterUK
  2. 2.Interface Analysis Centre, H.H. Wills Physics LaboratoryUniversity of BristolBristolUK
  3. 3.Biophotonics Research UnitGloucestershire Hospitals NHS Foundation TrustGloucesterUK

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