Analytical and Bioanalytical Chemistry

, Volume 407, Issue 27, pp 8279–8289 | Cite as

Raman spectroscopy for screening and diagnosis of cervical cancer

  • Fiona M. LyngEmail author
  • Damien Traynor
  • Inês R. M. Ramos
  • Franck Bonnier
  • Hugh J. Byrne
Part of the following topical collections:
  1. Raman4Clinics


Cervical cancer is the fourth most common cancer in women worldwide and mainly affects younger women. The mortality associated with cervical cancer can be reduced if the disease is detected at the pre-cancer stage. Current best-practice methods include cytopathology, HPV testing, and histopathology, but these methods are limited in terms of subjectivity, cost, and time. There is an unmet clinical need for new methods to aid clinicians in the early detection of cervical pre-cancer. These methods should be objective and rapid and require minimal sample preparation. Raman spectroscopy is a vibrational spectroscopic technique by which incident radiation is used to induce vibrations in the molecules of a sample and the scattered radiation may be used to characterise the sample in a rapid and non-destructive manner. Raman spectroscopy is sensitive to subtle biochemical changes occurring at the molecular level, enabling spectral variations corresponding to disease onset to be detected. Over the past 15 years, there have been numerous reports revealing the potential of Raman spectroscopy together with multivariate statistical analysis for the detection of a variety of cancers. This paper discusses the recent advances and challenges for cervical-cancer screening and diagnosis and offers some perspectives for the future.


Raman spectroscopy Cervical cancer Cervical intraepithelial neoplasia (CIN) Low-grade squamous intraepithelial lesion (LSIL) High-grade squamous intraepithelial lesion (HSIL) Cytopathology Histopathology Human papilloma virus (HPV) 



The authors acknowledge funding from Enterprise Ireland co-funded by the European Regional Development Fund (ERDF) and Ireland’s EU Structural Funds Programme 2007–2013, CF2011 1045, the Health Research Board Collaborative Applied Research Grant, CARG2012/29, and Dublin Institute of Technology Fiosraigh Research Excellence Award.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Fiona M. Lyng
    • 1
    • 2
    Email author
  • Damien Traynor
    • 1
    • 2
  • Inês R. M. Ramos
    • 1
    • 2
  • Franck Bonnier
    • 3
    • 4
  • Hugh J. Byrne
    • 3
  1. 1.DIT Centre for Radiation and Environmental ScienceFOCAS Research Institute, Dublin Institute of TechnologyDublin 8Ireland
  2. 2.School of PhysicsDublin Institute of TechnologyDublin 8Ireland
  3. 3.FOCAS Research InstituteDublin Institute of TechnologyDublin 8Ireland
  4. 4.Faculty of Pharmacy, EA 6295 Nanomédicaments et NanosondesUniversité François-Rabelais de ToursToursFrance

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