Raman spectroscopy for screening and diagnosis of cervical cancer
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Cervical cancer is the fourth most common cancer in women worldwide and mainly affects younger women. The mortality associated with cervical cancer can be reduced if the disease is detected at the pre-cancer stage. Current best-practice methods include cytopathology, HPV testing, and histopathology, but these methods are limited in terms of subjectivity, cost, and time. There is an unmet clinical need for new methods to aid clinicians in the early detection of cervical pre-cancer. These methods should be objective and rapid and require minimal sample preparation. Raman spectroscopy is a vibrational spectroscopic technique by which incident radiation is used to induce vibrations in the molecules of a sample and the scattered radiation may be used to characterise the sample in a rapid and non-destructive manner. Raman spectroscopy is sensitive to subtle biochemical changes occurring at the molecular level, enabling spectral variations corresponding to disease onset to be detected. Over the past 15 years, there have been numerous reports revealing the potential of Raman spectroscopy together with multivariate statistical analysis for the detection of a variety of cancers. This paper discusses the recent advances and challenges for cervical-cancer screening and diagnosis and offers some perspectives for the future.
KeywordsRaman spectroscopy Cervical cancer Cervical intraepithelial neoplasia (CIN) Low-grade squamous intraepithelial lesion (LSIL) High-grade squamous intraepithelial lesion (HSIL) Cytopathology Histopathology Human papilloma virus (HPV)
The authors acknowledge funding from Enterprise Ireland co-funded by the European Regional Development Fund (ERDF) and Ireland’s EU Structural Funds Programme 2007–2013, CF2011 1045, the Health Research Board Collaborative Applied Research Grant, CARG2012/29, and Dublin Institute of Technology Fiosraigh Research Excellence Award.
- 1.Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F (2012) GLOBOCAN, v1.0, Cancer incidence and mortality worldwide: IARC Cancer Base No. 11, 2013. International Agency for Research on CancerGoogle Scholar
- 4.Koss LG, Melamed MR (2006) Koss’ diagnostic cytology and its histopathologic bases, 5th edn. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
- 12.Dalla Palma P, Giorgi Rossi P, Collina G, Buccoliero AM, Ghiringhello B, Lestani M et al (2008) The risk of false-positive histology according to the reason for colposcopy referral in cervical cancer screening: a blind revision of all histologic lesions found in the NTCC trial. Am J Clin Pathol 129:75–80CrossRefGoogle Scholar
- 16.Diem M (1993) Introduction to modern vibrational spectroscopy. Wiley, New YorkGoogle Scholar
- 22.Rashid N (2013) Raman microspectroscopy for the characterization of cervical cancer, PhD thesis, Dublin Institute of TechnologyGoogle Scholar
- 24.Kerr LT, Byrne HJ, Hennelly BM (2015) Optimal choice of sample substrate and laser wavelength for Raman spectroscopic analysis of biological specimens. Anal Methods (accepted)Google Scholar