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Glucaminium ionic liquid-functionalized stationary phase for the separation of nucleosides in hydrophilic interaction chromatography

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Abstract

A glucaminium-based ionic liquid stationary phase was prepared via facile epoxy-amine reaction and subsequent quaternization. Successful immobilization of glucaminium-based ionic liquid onto silica surface was validated by elemental analysis and infrared spectroscopy. The new stationary phase was evaluated for the separation of nucleosides in hydrophilic interaction liquid chromatography (HILIC). Effects of various factors, such as acetonitrile concentration, salt concentration, pH value, as well as column temperature, on the chromatographic behavior toward nucleosides were studied in detail. The results indicated that this new stationary phase can be used for separation of water-soluble polar substances in HILIC mode. The retention of solutes on the stationary phase was influenced by a mixed-mode retention mechanism with a combination of adsorptive and partitioning interactions.

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References

  1. Welton T (1999) Chem Rev 99:2071–2083

    Article  CAS  Google Scholar 

  2. Welton T (2008) Ionic liquids in synthesis, Wiley Online Library

  3. Olivier-Bourbigou H, Magna L (2002) J Mol Catal A Chem 182:419–437

    Article  Google Scholar 

  4. Tunckol M, Durand J, Serp P (2012) Carbon 50(12):4303–4334

    Article  CAS  Google Scholar 

  5. Liu J, Jiang G, Liu J, Jönsson JÅ (2005) TrAC Trends Anal Chem 24:20–27

    Article  Google Scholar 

  6. Sun P, Armstrong DW (2010) Anal Chim Acta 661:1–16

    Article  CAS  Google Scholar 

  7. López-Darias J, Germán-Hernández M, Pino V, Afonso AM (2010) Talanta 80:1611–1618

    Article  Google Scholar 

  8. Ho TD, Zhang C, Hantao LW, Anderson JL (2014) Anal Chem 86:262–285

    Article  CAS  Google Scholar 

  9. Zhou Q, Bai H, Xie G, Xiao J (2008) J Chromatogr A 1177:43–49

    Article  CAS  Google Scholar 

  10. Vidal L, Riekkola M-L, Canals A (2012) Anal Chim Acta 715:19–41

    Article  CAS  Google Scholar 

  11. Yu H, Ho TD, Anderson JL (2013) Trends Anal Chem 45:219–232

    Article  CAS  Google Scholar 

  12. Ding J, Welton T, Armstrong DW (2004) Anal Chem 76:6819–6822

    Article  CAS  Google Scholar 

  13. Anderson JL, Armstrong DW (2003) Anal Chem 75:4851–4858

    Article  CAS  Google Scholar 

  14. Xiao XH, Zhao L, Liu X, Jiang SX (2004) Anal Chim Acta 519:207–211

    Article  CAS  Google Scholar 

  15. Wang Q, Baker GA, Baker SN, Colon LA (2006) Analyst 131:1000–1005

    Article  CAS  Google Scholar 

  16. Berthod A, Ruiz-Ángel MJ, Carda-Broch S (2008) J Chromatogr A 1184:6–18

    Article  CAS  Google Scholar 

  17. Bi W, Zhou J, Row K-H (2012) Separ Sci Technol 47:360–369

    Article  CAS  Google Scholar 

  18. Pino V, Afonso AM (2012) Anal Chim Acta 714:20–37

    Article  CAS  Google Scholar 

  19. Zhang M, Liang X, Jiang S, Qiu H (2014) TrAC Trends Anal Chem 53:60–72

    Article  CAS  Google Scholar 

  20. Joshi MD, Chalumot G, Kim Y-W, Anderson JL (2012) Chem Commun 48:1410–1412

    Article  CAS  Google Scholar 

  21. Joshi MD, Li T, Zhong Q, Anderson JL (2013) J Chromatogr A 1308:161–165

    Article  CAS  Google Scholar 

  22. Qiu H, Liang X, Sun M, Jiang S (2011) Anal Bioanal Chem 399:3307–3322

    Article  CAS  Google Scholar 

  23. Joshi MD, Steyer DJ, Anderson JL (2012) Anal Chim Acta 740:66–73

    Article  CAS  Google Scholar 

  24. Ho TD, Joshi MD, Silver MA, Anderson JL (2012) J Chromatogr A 1240:29–44

    Article  CAS  Google Scholar 

  25. Qiao L, Wang S, Li H, Shan YH, Dou AB, Shi XZ, Xu GW (2014) J Chromatogr A 1360:240–247

    Article  CAS  Google Scholar 

  26. Warwel S, Klaas MR, Schier H, Brüse F, Wiege B (2001) Eur J Lipid Sci Technol 103:645–654

    Article  CAS  Google Scholar 

  27. Qiu H, Mallik AK, Takafuji M, Jiang S, Ihara H (2012) RSC Adv 137:2553–2555

    CAS  Google Scholar 

  28. Zhang ML, Chen J, Gua TN, Qiu HD, Jiang SX (2014) Talanta 126:177–184

    Article  CAS  Google Scholar 

  29. Qiu H, Mallik AK, Takafuji M, Liu X, Jiang S, Ihara H (2012) J Chromatogr A 1232:116–122

    Article  CAS  Google Scholar 

  30. Jia J, Liu S, Qiu H, Guo Y, Liu X, Jiang S (2013) Anal Bioanal Chem 406:2163–2170

    Article  Google Scholar 

  31. Murray F (1995) Regul Toxicol Pharmacol 22:221–230

    Article  CAS  Google Scholar 

  32. Stahlberg J (1999) J Chromatogr A 855:3–55

    Article  CAS  Google Scholar 

  33. Alpert AJ (2008) Anal Chem 80:62–76

    Article  CAS  Google Scholar 

  34. McCalley DV (2010) J Chromatogr A 1217:3408–3417

    Article  CAS  Google Scholar 

  35. Qiao L, Dou A, Shi X, Li H, Shan Y, Lu X, Xu G (2013) J Chromatogr A 1286:137–145

    Article  CAS  Google Scholar 

  36. Chirita RI, West C, Zubrzycki S, Finaru AL, Elfakir C (2011) J Chromatogr A 1218:5939–5963

    Article  CAS  Google Scholar 

  37. Rodriguez-Gonzalo E, Garcia-Gomez D, Carabias-Martinez R (2011) J Chromatogr A 1218:9055–9063

    Article  CAS  Google Scholar 

  38. Molnár-Perl I (2000) J Chromatogr A 891:1–32

    Article  Google Scholar 

  39. Guo Y, Gaiki S (2005) J Chromatogr A 1074:71–80

    Article  CAS  Google Scholar 

  40. Sun M, Feng J, Luo C, Liu X, Jiang S (2014) Anal Bioanal Chem 406:2651–2658

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors express their thanks to the support of the “Hundred Talents Program” of Chinese Academy of Sciences, and the National Natural Science Foundation of China (No. 21475142).

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Authors and Affiliations

  1. Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou, 450052, China

    Qiong Jiang & Shusheng Zhang

  2. Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Mingliang Zhang, Xusheng Wang, Yong Guo & Hongdeng Qiu

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  1. Qiong Jiang
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  2. Mingliang Zhang
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  3. Xusheng Wang
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  4. Yong Guo
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  5. Hongdeng Qiu
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Correspondence to Hongdeng Qiu or Shusheng Zhang.

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Jiang, Q., Zhang, M., Wang, X. et al. Glucaminium ionic liquid-functionalized stationary phase for the separation of nucleosides in hydrophilic interaction chromatography. Anal Bioanal Chem 407, 7667–7672 (2015). https://doi.org/10.1007/s00216-015-8927-4

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  • DOI: https://doi.org/10.1007/s00216-015-8927-4

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