Analytical and Bioanalytical Chemistry

, Volume 407, Issue 25, pp 7667–7672 | Cite as

Glucaminium ionic liquid-functionalized stationary phase for the separation of nucleosides in hydrophilic interaction chromatography

  • Qiong Jiang
  • Mingliang Zhang
  • Xusheng Wang
  • Yong Guo
  • Hongdeng QiuEmail author
  • Shusheng ZhangEmail author
Research Paper


A glucaminium-based ionic liquid stationary phase was prepared via facile epoxy-amine reaction and subsequent quaternization. Successful immobilization of glucaminium-based ionic liquid onto silica surface was validated by elemental analysis and infrared spectroscopy. The new stationary phase was evaluated for the separation of nucleosides in hydrophilic interaction liquid chromatography (HILIC). Effects of various factors, such as acetonitrile concentration, salt concentration, pH value, as well as column temperature, on the chromatographic behavior toward nucleosides were studied in detail. The results indicated that this new stationary phase can be used for separation of water-soluble polar substances in HILIC mode. The retention of solutes on the stationary phase was influenced by a mixed-mode retention mechanism with a combination of adsorptive and partitioning interactions.


Ionic liquid Glucaminium∙stationary phase Hydrophilic interaction High-performance liquid chromatography 



The authors express their thanks to the support of the “Hundred Talents Program” of Chinese Academy of Sciences, and the National Natural Science Foundation of China (No. 21475142).

Supplementary material

216_2015_8927_MOESM1_ESM.pdf (198 kb)
ESM 1 (PDF 197 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of HenanZhengzhou UniversityZhengzhouChina
  2. 2.Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhouChina

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