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Effect of minimizing amount of template by addition of macromolecular crowding agent on preparation of molecularly imprinted monolith

Analytical and Bioanalytical Chemistry volume 407, pages7401–7412(2015)Cite this article

Abstract

One of the main challenges in the preparation of molecularly imprinted polymers (MIPs) is the substantial initial amount of template needed because of the requirement of high load capacities for most applications. A new strategy of macromolecular crowding was suggested to solve this problem by reducing the amount of template in the polymerization recipe. In a ternary porogenic system of polystyrene (PS) (crowding agent), tetrahydrofuran, and toluene, an imprinted monolithic column with high porosity and good permeability was synthesized using a mixture of ellagic acid (template), acrylamide, and ethylene glycol dimethacrylate. The effect of polymerization factors, including monomer–template molar ratio and the molecular weight and concentration of PS, on the imprinting effect of the resulting MIP monoliths was systematically investigated. At a high ratio of monomer–template (120:1), the greatest imprinting factor of 32.4 was obtained on the MIP monolith with the aid of macromolecular crowding agent. The PS-based imprinted monolith had imprinting even at the extremely high ratio of functional monomer to template of 1510:1. Furthermore, an off-line solid-phase extraction based on the ground MIP was conducted, and the purification recovery of ellagic acid from pomegranate-rind extract was up to 80 %. In conclusion, this approach based on macromolecular crowding is simple, and is especially valuable for those applications of MIP preparation for which a rare template is used.

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Acknowledgements

This work was financially supported by the High Technology Research and Development Program of Xinjiang (No. 201217149).

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Affiliations

  1. Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 40-1 South Beijing Road, Urumqi, 830011, Xinjiang, China
    • Guang-Ying Sun
    • , Dan-Dan Zhong
    • , Xiang-Jie Li
    • , Yu-Qing Luo
    • , Hang Ba
    • , Zhao-Sheng Liu
    •  & Haji Akber Aisa
  2. State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical en Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, Xinjiang, China
    • Guang-Ying Sun
    • , Dan-Dan Zhong
    • , Xiang-Jie Li
    • , Yu-Qing Luo
    • , Hang Ba
    • , Zhao-Sheng Liu
    •  & Haji Akber Aisa
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  1. Guang-Ying Sun
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Correspondence to Zhao-Sheng Liu or Haji Akber Aisa.

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Sun, G., Zhong, D., Li, X. et al. Effect of minimizing amount of template by addition of macromolecular crowding agent on preparation of molecularly imprinted monolith. Anal Bioanal Chem 407, 7401–7412 (2015). https://doi.org/10.1007/s00216-015-8902-0

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Keywords

  • Monolith
  • Molecularly imprinted polymer
  • Polystyrene
  • Ellagic acid
  • Macromolecular crowding agent