Analytical and Bioanalytical Chemistry

, Volume 407, Issue 21, pp 6381–6389 | Cite as

Structural characterization of major soyasaponins in traditional cultivars of Fagioli di Sarconi beans investigated by high-resolution tandem mass spectrometry

  • Giuliana BiancoEmail author
  • Alessandro Buchicchio
  • Tommaso R. I. Cataldi
Research Paper
Part of the following topical collections:
  1. High-Resolution Mass Spectrometry in Food and Environmental Analysis


Major soyasaponins, i.e., soyasaponins I, V, βg, and αg from traditional Fagioli di Sarconi beans (Phaseolus vulgaris L., ecotype Tabacchino), were analyzed by reversed-phase liquid chromatography–mass spectrometry (MS) using high-resolution Fourier transform ion cyclotron resonance (FTICR) MS on electrospray ionization in positive-ion mode. Fagioli di Sarconi beans are protected by the European Union [Commission Regulation (EC) No 1263/96] with the mark PGI (for “Protected Geographical Indication”), and are cultivated in Basilicata (southern Italy). Protonated adducts of soyasaponins I, V, βg, and αg were observed at m/z 943.5262, 959.5213, 1069.5583, and 1085.5534, respectively. Gas-phase dissociation of soyasaponins by infrared multiphoton dissociation FTICR MS was performed using a CO2 laser source at a wavelength of 10.6 μm. Most of the fragment ions were identified unambiguously by using the high-resolution and accurate mass value provided by the FTICR mass spectrometer. All soyasaponins exhibit a sequential and neutral loss of sugar moieties at relatively short irradiation times (i.e., less than 50 ms). When the pulse length was increased, a more pronounced fragmentation occurred, with several signals in the lower part of the mass spectrum. In the case of soyasaponins βg and αg, the occurrence of the conjugated product ion at m/z 127.0389 ([C6H6O3 + H]+, 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) was evidenced. Coupling reversed-phase liquid chromatography with high-performance FTICR MS in combination with infrared multiphoton dissociation tandem MS proved to be very promising for the structural characterization of soyasaponins, and is also suitable for the rapid and accurate structural investigation of other saponins.

Graphical Abstract

Representative Infrared Multiphoton Dissociation (IRMPD)–FTICR MS spectra of main group B saponins in Fagioli di Sarconi beans


Group B soyasaponins Soyasaponins I βg αg Infrared multiphoton dissociation Mass spectrometry Ion cyclotron resonance Mass accuracy 



This work was performed by using the instrumental facilities of the CIGAS Center supported by the EU (project no. 2915/12), Regione Basilicata, and Università degli Studi della Basilicata.

Supplementary material

216_2015_8810_MOESM1_ESM.pdf (54 kb)
ESM 1 (PDF 53 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Giuliana Bianco
    • 1
    Email author
  • Alessandro Buchicchio
    • 2
  • Tommaso R. I. Cataldi
    • 3
    • 4
  1. 1.Dipartimento di ScienzeUniversità degli Studi della BasilicataPotenzaItaly
  2. 2.Scuola di IngegneriaUniversità degli Studi della BasilicataPotenzaItaly
  3. 3.Dipartimento di ChimicaUniversità degli Studi di Bari Aldo MoroBariItaly
  4. 4.Centro Interdipartimentale SMARTUniversità degli Studi di Bari Aldo MoroBariItaly

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