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Structural characterization of major soyasaponins in traditional cultivars of Fagioli di Sarconi beans investigated by high-resolution tandem mass spectrometry

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Abstract

Major soyasaponins, i.e., soyasaponins I, V, βg, and αg from traditional Fagioli di Sarconi beans (Phaseolus vulgaris L., ecotype Tabacchino), were analyzed by reversed-phase liquid chromatography–mass spectrometry (MS) using high-resolution Fourier transform ion cyclotron resonance (FTICR) MS on electrospray ionization in positive-ion mode. Fagioli di Sarconi beans are protected by the European Union [Commission Regulation (EC) No 1263/96] with the mark PGI (for “Protected Geographical Indication”), and are cultivated in Basilicata (southern Italy). Protonated adducts of soyasaponins I, V, βg, and αg were observed at m/z 943.5262, 959.5213, 1069.5583, and 1085.5534, respectively. Gas-phase dissociation of soyasaponins by infrared multiphoton dissociation FTICR MS was performed using a CO2 laser source at a wavelength of 10.6 μm. Most of the fragment ions were identified unambiguously by using the high-resolution and accurate mass value provided by the FTICR mass spectrometer. All soyasaponins exhibit a sequential and neutral loss of sugar moieties at relatively short irradiation times (i.e., less than 50 ms). When the pulse length was increased, a more pronounced fragmentation occurred, with several signals in the lower part of the mass spectrum. In the case of soyasaponins βg and αg, the occurrence of the conjugated product ion at m/z 127.0389 ([C6H6O3 + H]+, 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) was evidenced. Coupling reversed-phase liquid chromatography with high-performance FTICR MS in combination with infrared multiphoton dissociation tandem MS proved to be very promising for the structural characterization of soyasaponins, and is also suitable for the rapid and accurate structural investigation of other saponins.

Representative Infrared Multiphoton Dissociation (IRMPD)–FTICR MS spectra of main group B saponins in Fagioli di Sarconi beans

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Acknowledgments

This work was performed by using the instrumental facilities of the CIGAS Center supported by the EU (project no. 2915/12), Regione Basilicata, and Università degli Studi della Basilicata.

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Authors and Affiliations

  1. Dipartimento di Scienze, Università degli Studi della Basilicata, Via dell’Ateneo Lucano 10, 85100, Potenza, Italy

    Giuliana Bianco

  2. Scuola di Ingegneria, Università degli Studi della Basilicata, Via dell’Ateneo Lucano 10, 85100, Potenza, Italy

    Alessandro Buchicchio

  3. Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, Campus Universitario, Via E. Orabona 4, 70126, Bari, Italy

    Tommaso R. I. Cataldi

  4. Centro Interdipartimentale SMART, Università degli Studi di Bari Aldo Moro, Campus Universitario, Via E. Orabona 4, 70126, Bari, Italy

    Tommaso R. I. Cataldi

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  1. Giuliana Bianco
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  2. Alessandro Buchicchio
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  3. Tommaso R. I. Cataldi
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Correspondence to Giuliana Bianco.

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Published in the topical collection High-Resolution Mass Spectrometry in Food and Environmental Analysis with guest editor Aldo Laganà.

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Bianco, G., Buchicchio, A. & Cataldi, T.R.I. Structural characterization of major soyasaponins in traditional cultivars of Fagioli di Sarconi beans investigated by high-resolution tandem mass spectrometry. Anal Bioanal Chem 407, 6381–6389 (2015). https://doi.org/10.1007/s00216-015-8810-3

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  • DOI: https://doi.org/10.1007/s00216-015-8810-3

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