Analytical and Bioanalytical Chemistry

, Volume 407, Issue 27, pp 8291–8301

Combined fiber probe for fluorescence lifetime and Raman spectroscopy

  • Sebastian Dochow
  • Dinglong Ma
  • Ines Latka
  • Thomas Bocklitz
  • Brad Hartl
  • Julien Bec
  • Hussain Fatakdawala
  • Eric Marple
  • Kirk Urmey
  • Sebastian Wachsmann-Hogiu
  • Michael Schmitt
  • Laura Marcu
  • Jürgen Popp
Paper in Forefront

DOI: 10.1007/s00216-015-8800-5

Cite this article as:
Dochow, S., Ma, D., Latka, I. et al. Anal Bioanal Chem (2015) 407: 8291. doi:10.1007/s00216-015-8800-5
Part of the following topical collections:
  1. Raman4Clinics

Abstract

In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated.

Graphical Abstract

An image comparison between FLIm and Raman spectroscopy acquired with the bimodal probe onseveral tissue samples

Keywords

Fluorescence lifetime Raman spectroscopy Bimodal Imaging Fiber probes 

Supplementary material

216_2015_8800_MOESM1_ESM.pdf (80 kb)
ESM 1(PDF 79 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sebastian Dochow
    • 1
  • Dinglong Ma
    • 2
  • Ines Latka
    • 1
  • Thomas Bocklitz
    • 1
  • Brad Hartl
    • 2
  • Julien Bec
    • 2
  • Hussain Fatakdawala
    • 2
  • Eric Marple
    • 4
  • Kirk Urmey
    • 4
  • Sebastian Wachsmann-Hogiu
    • 3
  • Michael Schmitt
    • 1
  • Laura Marcu
    • 2
  • Jürgen Popp
    • 1
    • 5
  1. 1.Institute of Physical Chemistry and Abbe Center of PhotonicsFriedrich Schiller University JenaJenaGermany
  2. 2.Department of Biomedical EngineeringUniversity of California, DavisDavisUSA
  3. 3.Department of Pathology and Laboratory Medicine and Center for BiophotonicsUniversity of California, DavisSacramentoUSA
  4. 4.EmVision LCCLoxahatcheeUSA
  5. 5.Leibniz Institute of Photonic Technology Jena e.V.JenaGermany

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