Combined fiber probe for fluorescence lifetime and Raman spectroscopy
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In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated.
KeywordsFluorescence lifetime Raman spectroscopy Bimodal Imaging Fiber probes
Current study was supported in part by National Institute of Health grants R21 CA178578 and R01 CA187427. Financial support of the Bundesministerium für Bildung und Forschung for the project “Fiber Health Probe” is highly acknowledged (FKZ: 13N1225, 3N12526), as well as the financial sponsorship of the Carl-Zeiss-Stiftung.
Compliance with ethical standards
All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. The Illinois Institutional Animal Care and Use Committee (IACUC) guidelines of all University of California institutions were followed.
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