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G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

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Abstract

Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) n stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak Gox) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak GHMDE was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) n stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Further measurements made by cyclic and alternating-current voltammetry revealed a strong influence of the ODN structure on their behavior at electrically charged surfaces.

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References

  1. Paleček E (1958) Oszillographische polarographie der nucleinsäuren und ihrer bestandteile. Naturwiss 45:186–187

    Article  Google Scholar 

  2. Paleček E, Fojta M, Jelen F, Vetterl V (2002) Electrochemical analysis of nucleic acids. In: Bard AJ, Stratsmann M (eds) The encyclopedia of electrochemistry, Bioelectrochemistry, vol 9. Wiley-VCH, Weinheim

    Google Scholar 

  3. Paleček E, Jelen F (2005) In: Paleček E, Scheller F, Wang J (eds) Electrochemistry of nucleic acids and proteins Towards electrochemical sensors for genomics and proteomics vol 1. Elsevier, Amsterdam

    Google Scholar 

  4. Studničková M, Trnková L, Zetek J, Glatz Z (1989) Reduction of guanosine at a mercury-electrode. Bioelectrochem Bioenerg 21:83–86

    Article  Google Scholar 

  5. Trnková L, Studničková M, Paleček E (1980) Electrochemical behaviour of guanine and its derivatives. I.Fast cyclic voltammetry of guanosine and calf thymus DNA. Bioelectrochem Bioenerg 7:644–658

    Article  Google Scholar 

  6. Paleček E, Bartošík M (2012) Electrochemistry of Nucleic Acids. Chem Rev 112:3427–3481

    Article  Google Scholar 

  7. Fojta M, Palecek E (1997) Supercoiled DNA-modified mercury electrode: A highly sensitive tool for the detection of DNA damage. Anal Chim Acta 342:1–12

    Article  CAS  Google Scholar 

  8. Fojta M, Havran L, Fulneckova J, Kubicarova T (2000) Adsorptive transfer stripping AC voltammetry of DNA complexes with intercalators. Electroanalysis 12:926–934

    Article  CAS  Google Scholar 

  9. Fojta M, Bowater RP, Stankova V, Havran L, Lilley DMJ, Palecek E (1998) Two superhelix density-dependent DNA transitions detected by changes in DNA adsorption/desorption behavior. 37:4853–4862

  10. Wang J (2002) Electrochemical nucleic acid biosensors. Anal Chim Acta 469:63–71

    Article  CAS  Google Scholar 

  11. Drummond TG, Hill MG, Barton JK (2003) Electrochemical DNA sensors. Nat Biotechnol 21:1192–1199

    Article  CAS  Google Scholar 

  12. Trnková L, Postbieglová I, Holík M (2004) Electroanalytical determination of d(GCGAAGC) hairpin. Bioelectrochemistry 63:25–30

    Article  Google Scholar 

  13. Gorodetsky AA, Buzzeo MC, Barton JK (2008) DNA-Mediated Electrochemistry. Bioconjug Chem 19:2285–2296

    Article  CAS  Google Scholar 

  14. Hason S, Vetterl V, Fojta M (2008) Two-dimensional condensation of pyrimidine oligonucleotides during their self-assemblies at mercury based surfaces. Electrochim Acta 53:2818–2824

    Article  CAS  Google Scholar 

  15. Mikelova R, Trnkova L, Jelen F (2007) Double elimination voltammetry of short oligonucleotides. Electroanalysis 19:1807–1814

    Article  CAS  Google Scholar 

  16. Mikelova R, Trnkova L, Jelen F, Adam V, Kizek R (2007) Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry. Electroanalysis 19:348–355

    Article  CAS  Google Scholar 

  17. Pilarova I, Kejnovska I, Vorlickova M, Trnkova L (2014) Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods. Electroanalysis 26:2118–2128

    Article  CAS  Google Scholar 

  18. Trnková L, Jelen F, Postbieglová I (2006) Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides. Electroanalysis 18:662–669

    Article  Google Scholar 

  19. Chiorcea-Paquim AM, Viegas Santos P, Eritja R, Oliveira-Brett AM (2013) Self-assembled G-quadruplex nanostructures: AFM and voltammetric characterization. Phys Chem Chem Phys 15:9117–9124

    Article  CAS  Google Scholar 

  20. Chiorcea-Paquim A-M, Oliveira-Brett AM (2014) Redox behaviour of G-quadruplexes. Electrochim Acta 126:162–170

    Article  CAS  Google Scholar 

  21. Chiorcea-Paquim A-M, Santos PV, Oliveira-Brett AM (2013) Atomic force microscopy and voltammetric characterisation of synthetic homo-oligodeoxynucleotides. Electrochim Acta 110:599–607

    Article  CAS  Google Scholar 

  22. Fojta M, Vetterl V, Tomschik M, Jelen F, Nielsen P, Wang J, Palecek E (1997) Adsorption of peptide nucleic acid and DNA decamers at electrically charged surfaces. Biophys J 72:2285–2293

    Article  CAS  Google Scholar 

  23. Tomschik M, Jelen F, Havran L, Trnková L, Nielsen PE, Paleček E (1999) Reduction and oxidation of peptide nucleic acids and DNA at mercury and carbon electrodes. J Electroanal Chem 476:71–80

    Article  CAS  Google Scholar 

  24. Sambrook J, Fritsch EF, Maniatis T (1987) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press:9.36

  25. Fojta M, Jelen F, Havran L, Palecek E (2008) Electrochemical stripping techniques in analysis of nucleic acids and their constituents. Curr Anal Chem 4:250–262

    Article  CAS  Google Scholar 

  26. Cai XH, Rivas G, Shirashi H, Farias P, Wang J, Tomschik M, Jelen F, Palecek E (1997) Electrochemical analysis of formation of polynucleotide complexes in solution and at electrode surfaces. Anal Chim Acta 344:65–76

    Article  CAS  Google Scholar 

  27. Palecek E (1991) Local supercoil-stabilized DNA structures. Crit Rev Biochem Mol Biol 26:151–226

    Article  CAS  Google Scholar 

  28. Kypr J, Kejnovská I, Renčiuk D, Vorlíčková M (2009) Circular dichroism and conformational polymorphism of DNA. Nucleic Acids Res 37:1713–1725

    Article  CAS  Google Scholar 

  29. Rodrigues Pontinha AD, Chiorcea-Paquim A-M, Eritja R, Oliveira-Brett AM (2014) Quadruplex Nanostructures of d(TGGGGT): Influence of Sodium and Potassium Ions. Anal Chem 86:5851–5857

    Article  CAS  Google Scholar 

  30. Palecek E, Jelen F, Trnkova L (1986) Cyclic voltammetry of DNA at a mercury-electrode - an anodic peak specific for guanine. Gen Physiol Biophys 5:315–329

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants of the Czech Science Foundation (P206/12/2378 to L.H.) and by the ASCR (RVO 68081707).

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Authors and Affiliations

  1. Institute of Biophysics of the Academy of Sciences of the Czech Republic v.v.i., Kralovopolska 135, 61265, Brno, Czech Republic

    Pavlína Vidláková, Hana Pivoňková, Iva Kejnovská, Michaela Vorlíčková, Miroslav Fojta & Luděk Havran

  2. CEITEC-Central European Institute of Technology, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Iva Kejnovská, Michaela Vorlíčková, Miroslav Fojta & Luděk Havran

  3. Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Libuše Trnková

  4. CEITEC, Brno University of Technology, Technicka 3058/10, 61600, Brno, Czech Republic

    Libuše Trnková

Authors
  1. Pavlína Vidláková
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  2. Hana Pivoňková
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  3. Iva Kejnovská
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  4. Libuše Trnková
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  5. Michaela Vorlíčková
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  6. Miroslav Fojta
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  7. Luděk Havran
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Correspondence to Miroslav Fojta or Luděk Havran.

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Vidláková, P., Pivoňková, H., Kejnovská, I. et al. G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques. Anal Bioanal Chem 407, 5817–5826 (2015). https://doi.org/10.1007/s00216-015-8768-1

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  • DOI: https://doi.org/10.1007/s00216-015-8768-1

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