Analytical and Bioanalytical Chemistry

, Volume 407, Issue 27, pp 8303–8310 | Cite as

Comparative study of the endoscope-based bevelled and volume fiber-optic Raman probes for optical diagnosis of gastric dysplasia in vivo at endoscopy

  • Jianfeng Wang
  • Kan Lin
  • Wei Zheng
  • Khek Yu Ho
  • Ming Teh
  • Khay Guan Yeoh
  • Zhiwei Huang
Research Paper
Part of the following topical collections:
  1. Raman4Clinics


This study aims to compare the diagnostic performance of the two different endoscope-based fiber-optic Raman probe designs (i.e., bevelled and volume Raman probes) for real-time, in vivo detection of gastric dysplasia at endoscopy. To conduct the clinical comparison, a total of 1,050 in vivo tissue Raman spectra (normal: n = 864; dysplasia: n = 186) were acquired from 66 gastric patients (normal: n = 48; dysplasia: n = 18) by using bevelled Raman probe, while a total of 1,913 in vivo tissue Raman spectra (normal: n = 1,786; dysplasia: n = 127) were acquired from 98 gastric patients (normal: n = 87; dysplasia: n = 11) by using volume Raman probe. The bevelled Raman probe provides approximately twofold improvements in tissue Raman-to-autofluorescence intensity ratios as compared to the use of volume Raman probe. Partial least squares discriminant analysis together with leave-one patient-out cross-validation on in vivo tissue Raman spectra acquired yields a diagnostic accuracy of 93.0 % (sensitivity of 92.5 %; specificity of 93.1 %) for differentiating gastric dysplasia from normal gastric tissue by using the bevelled fiber-optic Raman probe, which is superior to the diagnostic performance (accuracy of 88.4 %; sensitivity of 85.8 %; specificity of 88.6 %) by using the volume Raman probe. This work demonstrates that the Raman spectroscopic technique coupled with bevelled fiber-optic Raman probe has great potential to enhance in vivo diagnosis of gastric precancer and early cancer at endoscopy.

Graphical Abstract

Comparison of in vivo gastric tissue Raman spectra acquired by using bevelled and volume fiber-optic Raman probes


Raman spectroscopy Fiber-optic Raman probe In vivo optical diagnosis Gastric dysplasia Endoscopy 



This work was supported by the National Medical Research Council (NMRC) (NMRC/CIRG/1331/2012; BnB13dec037), the National Research Foundation-Proof-of-Concept (NRF-POC) (NRF2014NRF-POC001-012), and the Academic Research Fund (AcRF)-Tier 2 from the Ministry of Education (MOE) (MOE2014-T2-1-010), Singapore.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Jianfeng Wang
    • 1
  • Kan Lin
    • 1
  • Wei Zheng
    • 1
  • Khek Yu Ho
    • 2
  • Ming Teh
    • 3
  • Khay Guan Yeoh
    • 2
  • Zhiwei Huang
    • 1
  1. 1.Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of EngineeringNational University of SingaporeSingaporeSingapore
  2. 2.Department of Medicine, Yong Loo Lin School of MedicineNational University of Singapore and National University Health SystemSingaporeSingapore
  3. 3.Department of Pathology, Yong Loo Lin School of MedicineNational University of Singapore and National University Health SystemSingaporeSingapore

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