Skip to main content
Log in

Cyclic voltammetric acetylcholinesterase biosensor for the detection of captan in apple samples with the aid of chemometrics

  • Note
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

The presence of captan residues in apples shows high toxicity, which often causes eye and skin irritation, dermatitis, conjunctivitis, and vomiting in humans. In this context, an electrochemical biosensor based on acetylcholinesterase (AChE) immobilized on a ZnO nanorod interface has been proposed. In this work, Hill, dose-response, and first-, second-, and third-order polynomial regression models were successfully applied and the prediction ability of these models was tested with the use of current density obtained from the cyclic voltammograms of appropriate captan solutions. The Pt/ZnO/AChE bioelectrode showed a high sensitivity of 0.538 μA cm−2 μM−1 in the linear range from 0.05 to 25.0 μM with a limit of detection of 107 nM. The recovery results were observed between 98.4 and 102.4 % from the apple sample. This work provides a new promising tool for the detection of captan in apple samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  1. Smith FD, MacHardy WE (1984) Phytopathol 74:894–899

    Article  CAS  Google Scholar 

  2. Singh RP, Kim YJ, Oh BK, Choi JW (2009) Electrochem Commun 11:181–185

    Article  Google Scholar 

  3. Rawn DF, Quade SC, Shields JB, Conca G, Sun WF, Smith M, Fouquet A, Belanger A (2007) Food Addit Contam 24:149–155

    Article  CAS  Google Scholar 

  4. Chauhan N, Narang J, Pundir CS (2011) Int J Biol Macromol 49:923–929

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Department of Science & Technology, New Delhi, for their financial support (DST/TSG/PT/2008/28, SR/FST/ETI-284/2011 (C) and Nano Mission Council (No. SR/NM/PG-16/2007)). We also thank SASTRA University for financial support (TRR fund).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to John Bosco Balaguru Rayappan.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 1087 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nesakumar, N., Sethuraman, S., Krishnan, U.M. et al. Cyclic voltammetric acetylcholinesterase biosensor for the detection of captan in apple samples with the aid of chemometrics. Anal Bioanal Chem 407, 4863–4868 (2015). https://doi.org/10.1007/s00216-015-8687-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-015-8687-1

Keywords

Navigation