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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 17, pp 5125–5131 | Cite as

Quantitative profiling of endocannabinoids in lipoproteins by LC–MS/MS

  • Mesut Bilgin
  • Laura Bindila
  • Juergen Graessler
  • Andrej ShevchenkoEmail author
Research Paper
Part of the following topical collections:
  1. Lipidomics

Abstract

Endocannabinoids belong to a diverse family of endogenous lipid bioregulators acting as physiological ligands of cannabinoid receptor type 1 and cannabinoid receptor type 2 in the central and peripheral nervous system. They are also present in nmol L−1 concentrations in human blood plasma; however, their association with possible molecular carriers remains poorly characterized. Here we report on the quantification of 46 endogenous molecular species from five major classes of endocannabinoids and endocannabinoid-related compounds in three lipoprotein fractions of human blood plasma: VLDL, LDL, HDL, and in the plasma lipoprotein-free fraction. Although sizable quantities of endocannabinoid-related molecules are associated with lipoproteins, we identified the lipoprotein-free fraction as a major carrier of endocannabinoids in blood circulation with the exception of 2-acylglycerols, which are markedly abundant in VLDL.

Keywords

Endocannabinoids Lipoproteins Blood plasma LC–MS/MS Quantification 

Notes

Acknowledgements

The authors are grateful for all members of AS laboratory for their experimental support and useful discussions. We are particularly indebted to Dr. Susanne Sales (MPI CBG) for her expert assistance with data processing and Sigrid Nitzsche (TUD) for lipoprotein preparation. Work in AS laboratory was supported by TRR83 grant (project A17) from Deutsche Forschungsgemeinschaft (DFG).

Supplementary material

216_2015_8559_MOESM1_ESM.pdf (112 kb)
ESM 1 (PDF 111 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Mesut Bilgin
    • 1
  • Laura Bindila
    • 2
  • Juergen Graessler
    • 3
  • Andrej Shevchenko
    • 1
    Email author
  1. 1.Max Planck Institute for Cell Biology and GeneticsDresdenGermany
  2. 2.Institute Physiological ChemistryUniversity Medical Center MainzMainzGermany
  3. 3.Department of Internal Medicine III, Medical Faculty Carl Gustav CarusTechnische Universität DresdenDresdenGermany

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