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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 17, pp 5113–5123 | Cite as

Nutrition-dependent changes of mouse adipose tissue compositions monitored by NMR, MS, and chromatographic methods

  • Yulia Popkova
  • Andrej Meusel
  • Jana Breitfeld
  • Dorit Schleinitz
  • Johannes Hirrlinger
  • Dirk Dannenberger
  • Peter Kovacs
  • Jürgen SchillerEmail author
Research Paper
Part of the following topical collections:
  1. Lipidomics

Abstract

Many diseases nowadays are assumed to be genetically determined. Therefore, many knockout mouse models have been established and are widely used. Unfortunately, nutrition (in particular the fat content of food) is often neglected in studies on these disease models. In this study the effects of nutrition on the lipid (triacylglycerol, TAG) compositions of different mouse adipose tissues were investigated. Mice were subjected to different diets [high fat (HF) vs. standard diet (SD)] and different adipose tissue samples (brown, visceral, and subcutaneous fat) were isolated after 12 weeks. Subsequent to lipid extraction, the organic extracts were analyzed by mass spectrometry (MALDI and ESI), high-resolution 1H and 31P NMR spectroscopy, high-performance thin-layer chromatography (HPTLC), and gas chromatography (GC). In adipose tissue of mice fed with HF diet, (a) decreased double bond contents and (b) decreased fatty acyl chain lengths of tissue TAGs were observed; this trend could be concomitantly monitored by all methods used. However, the adipose tissue still contained significant amounts of slightly unsaturated fatty acyl residues (18:1). Thus, a certain double bond content seems necessary to maintain the properties of adipose tissues.

Graphical Abstract

The compositions of different mouse adipose tissues is massively influenced by the composition of the supplied diet. This will be shown by using independent spectroscopic and chromatographic methods.

Keywords

Adipose tissue Triacylglycerols Phospholipids Double bond content MALDI MS NMR spectroscopy Gas chromatography 

Notes

Acknowledgments

This work was supported by the German Research Council (SFB 1052/B3 and B6). Dorit Schleinitz is funded by the Boehringer Ingelheim Foundation. We thank Dr. Beate Fuchs for performing the 31P NMR measurements and James Kranz for careful reading the manuscript.

Supplementary material

216_2015_8551_MOESM1_ESM.pdf (10 kb)
ESM 1 (PDF 9 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yulia Popkova
    • 1
  • Andrej Meusel
    • 1
  • Jana Breitfeld
    • 2
  • Dorit Schleinitz
    • 2
  • Johannes Hirrlinger
    • 3
    • 4
  • Dirk Dannenberger
    • 5
  • Peter Kovacs
    • 2
  • Jürgen Schiller
    • 1
    Email author
  1. 1.Medical Faculty, Institute of Medical Physics and BiophysicsUniversity of LeipzigLeipzigGermany
  2. 2.IFB Adiposity DiseasesUniversity of LeipzigLeipzigGermany
  3. 3.Medical Faculty, Carl-Ludwig-Institute for PhysiologyUniversity of LeipzigLeipzigGermany
  4. 4.Department of NeurogeneticsMax Planck Institute for Experimental MedicineGöttingenGermany
  5. 5.Leibniz Institute for Farm Animal BiologyInstitute of Muscle Biology and GrowthDummerstorfGermany

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