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Nutrition-dependent changes of mouse adipose tissue compositions monitored by NMR, MS, and chromatographic methods

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Abstract

Many diseases nowadays are assumed to be genetically determined. Therefore, many knockout mouse models have been established and are widely used. Unfortunately, nutrition (in particular the fat content of food) is often neglected in studies on these disease models. In this study the effects of nutrition on the lipid (triacylglycerol, TAG) compositions of different mouse adipose tissues were investigated. Mice were subjected to different diets [high fat (HF) vs. standard diet (SD)] and different adipose tissue samples (brown, visceral, and subcutaneous fat) were isolated after 12 weeks. Subsequent to lipid extraction, the organic extracts were analyzed by mass spectrometry (MALDI and ESI), high-resolution 1H and 31P NMR spectroscopy, high-performance thin-layer chromatography (HPTLC), and gas chromatography (GC). In adipose tissue of mice fed with HF diet, (a) decreased double bond contents and (b) decreased fatty acyl chain lengths of tissue TAGs were observed; this trend could be concomitantly monitored by all methods used. However, the adipose tissue still contained significant amounts of slightly unsaturated fatty acyl residues (18:1). Thus, a certain double bond content seems necessary to maintain the properties of adipose tissues.

The compositions of different mouse adipose tissues is massively influenced by the composition of the supplied diet. This will be shown by using independent spectroscopic and chromatographic methods.

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Acknowledgments

This work was supported by the German Research Council (SFB 1052/B3 and B6). Dorit Schleinitz is funded by the Boehringer Ingelheim Foundation. We thank Dr. Beate Fuchs for performing the 31P NMR measurements and James Kranz for careful reading the manuscript.

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    Authors and Affiliations

    1. Medical Faculty, Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstrasse 16-18, 04107, Leipzig, Germany

      Yulia Popkova, Andrej Meusel & Jürgen Schiller

    2. IFB Adiposity Diseases, University of Leipzig, Liebigstrasse 21, 04103, Leipzig, Germany

      Jana Breitfeld, Dorit Schleinitz & Peter Kovacs

    3. Medical Faculty, Carl-Ludwig-Institute for Physiology, University of Leipzig, Liebigstrasse 27, 04103, Leipzig, Germany

      Johannes Hirrlinger

    4. Department of Neurogenetics, Max Planck Institute for Experimental Medicine, Hermann-Rein-Str. 3, 37075, Göttingen, Germany

      Johannes Hirrlinger

    5. Leibniz Institute for Farm Animal Biology, Institute of Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

      Dirk Dannenberger

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    1. Yulia Popkova
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    Correspondence to Jürgen Schiller.

    Additional information

    Published in the topical collection Lipidomics with guest editor Michal Holčapek.

    Yulia Popkova and Andrej Meusel contributed equally to this work.

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    Popkova, Y., Meusel, A., Breitfeld, J. et al. Nutrition-dependent changes of mouse adipose tissue compositions monitored by NMR, MS, and chromatographic methods. Anal Bioanal Chem 407, 5113–5123 (2015). https://doi.org/10.1007/s00216-015-8551-3

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    • DOI: https://doi.org/10.1007/s00216-015-8551-3

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