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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 17, pp 5009–5020 | Cite as

Lipidome and metabolome analysis of fresh tobacco leaves in different geographical regions using liquid chromatography–mass spectrometry

  • Lili Li
  • Xin Lu
  • Jieyu Zhao
  • Junjie Zhang
  • Yanni Zhao
  • Chunxia ZhaoEmail author
  • Guowang Xu
Paper in Forefront
Part of the following topical collections:
  1. Lipidomics

Abstract

The combination of the lipidome and the metabolome can provide much more information in plant metabolomics studies. A method for the simultaneous extraction of the lipidome and the metabolome of fresh tobacco leaves was developed. Method validation was performed on the basis of the optimal ratio of methanol to methyl tert-butyl ether to water (37:45:68) from the design of experiments. Good repeatability was obtained. We found that 92.2 % and 91.6 % of the peaks for the lipidome and the metabolome were within a relative standard deviation of 20 %, accounting for 94.6 % and 94.6 % of the total abundance, respectively. The intraday and interday precisions were also satisfactory. A total of 230 metabolites, including 129 lipids, were identified. Significant differences were found in lipidomic and metabolomic profiles of fresh tobacco leaves in different geographical regions. Highly unsaturated galactolipids, phosphatidylethanolamines, predominant phosphatidylcholines, most of the polyphenols, amino acids, and polyamines had a higher content in Yunnan province, and low-unsaturation-degree galactolipids, triacylglycerols, glucosylceramides with trihydroxy long-chain bases, acylated sterol glucosides, and some organic acids were more abundant in Henan province. Correlation analysis between differential metabolites and climatic factors indicated the vital importance of temperature. The fatty acid unsaturation degree of galactolipids could be influenced by temperature. Accumulation of polyphenols and decreases in the ratios of stigmasterols to sitosterols and glucosylstigmasterols to glucosylsitosterols were also correlated with lower temperature in Yunnan province. Furthermore, lipids were more sensitive to climatic variations than other metabolites.

Graphical Abstract

Lipidomic and metabolomic profiles of tobacco in different regions

Keywords

Lipidome Metabolome Fresh tobacco leaves Climatic factors Metabolomics 

Notes

Acknowledgments

This study was supported by the Creative Research Group Project (no. 21321064) from National Natural Science Foundation of China.

Supplementary material

216_2015_8522_MOESM1_ESM.pdf (652 kb)
ESM 1 (PDF 652 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Lili Li
    • 1
  • Xin Lu
    • 1
  • Jieyu Zhao
    • 1
  • Junjie Zhang
    • 1
  • Yanni Zhao
    • 1
  • Chunxia Zhao
    • 1
    Email author
  • Guowang Xu
    • 1
  1. 1.Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

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