Raman spectroscopic investigation of 13CO2 labeling and leaf dark respiration of Fagus sylvatica L. (European beech)
- 306 Downloads
An important issue, in times of climate change and more extreme weather events, is the investigation of forest ecosystem reactions to these events. Longer drought periods stress the vitality of trees and promote mass insect outbreaks, which strongly affect ecosystem processes and services. Cavity-enhanced Raman gas spectrometry was applied for online multi-gas analysis of the gas exchange rates of O2 and CO2 and the labeling of Fagus sylvatica L. (European beech) seedlings with 13CO2. The rapid monitoring of all these gases simultaneously allowed for the separation of photosynthetic uptake of CO2 by the beech seedlings and a constant 12CO2 efflux via respiration and thus for a correction of the measured 12CO2 concentrations in course of the labeling experiment. The effects of aphid infestation with the woolly beech aphid (Phyllaphis fagi L.) as well as the effect of a drought period on the respirational gas exchange were investigated. A slightly decreased respirational activity of drought-stressed seedlings in comparison to normally watered seedlings was found already for a low drought intensity. Cavity-enhanced Raman gas monitoring of O2, 12CO2, and 13CO2 was proven to be a powerful new tool for studying the effect of drought stress and aphid infestation on the respirational activity of European beech seedlings as an example of important forest species in Central Europe.
KeywordsCavity-enhanced Raman spectroscopy Raman gas sensing 13CO2 labeling Environmental sensing Tree seedlings
Funding of the research project by the “ProExzellenz” program of the Free State of Thuringia, Germany, and by the Collaborative Research Centre 1076 “AquaDiva” from the Deutsche Forschungsgemeinschaft (DFG) is highly acknowledged. B.M. and M-C.G. thank the student workers for their assistance during the experimental work.
- 1.Michalzik B (2011) Insects Infestations Nutr Fluxes 216:557–580Google Scholar
- 2.Dale VH, Joyce LA, McNulty S, Neilson RP, Ayres MP, Flannigan MD, Hanson PJ, Irland LC, Lugo AE, Peterson CJ, Simberloff D, Swanson FJ, Stocks BJ, Michael Wotton B (2001) Climate change and forest disturbances. Bio Sci 51(9):723Google Scholar
- 4.Meinke I (2010) Klimawandel an der deutschen Ostseeküste. Meer und Küste, p 2Google Scholar
- 7.Pretzsch, H.; Schutze, G; Uhl, E (2012) Resistance of European tree species to drought stress in mixed versus pure forests: evidence of stress release by inter-specific facilitation. Plant Biol (Stuttg) Google Scholar
- 17.Hanf R, Bögözi T, Keiner R, Frosch T, Popp J (2015) Fast and highly sensitive fiber enhanced Raman spectroscopic monitoring of molecular H2 and CH4 for point-of-care diagnosis of malabsorption disorders in exhaled human breath. Anal Chem. doi: 10.1021/ac503450y