Analytical and Bioanalytical Chemistry

, Volume 407, Issue 7, pp 1813–1817 | Cite as

Raman spectroscopic investigation of 13CO2 labeling and leaf dark respiration of Fagus sylvatica L. (European beech)

  • Robert Keiner
  • Marie-Cécile Gruselle
  • Beate Michalzik
  • Jürgen Popp
  • Torsten FroschEmail author


An important issue, in times of climate change and more extreme weather events, is the investigation of forest ecosystem reactions to these events. Longer drought periods stress the vitality of trees and promote mass insect outbreaks, which strongly affect ecosystem processes and services. Cavity-enhanced Raman gas spectrometry was applied for online multi-gas analysis of the gas exchange rates of O2 and CO2 and the labeling of Fagus sylvatica L. (European beech) seedlings with 13CO2. The rapid monitoring of all these gases simultaneously allowed for the separation of photosynthetic uptake of CO2 by the beech seedlings and a constant 12CO2 efflux via respiration and thus for a correction of the measured 12CO2 concentrations in course of the labeling experiment. The effects of aphid infestation with the woolly beech aphid (Phyllaphis fagi L.) as well as the effect of a drought period on the respirational gas exchange were investigated. A slightly decreased respirational activity of drought-stressed seedlings in comparison to normally watered seedlings was found already for a low drought intensity. Cavity-enhanced Raman gas monitoring of O2, 12CO2, and 13CO2 was proven to be a powerful new tool for studying the effect of drought stress and aphid infestation on the respirational activity of European beech seedlings as an example of important forest species in Central Europe.


Cavity-enhanced Raman spectroscopy Raman gas sensing 13CO2 labeling Environmental sensing Tree seedlings 



Funding of the research project by the “ProExzellenz” program of the Free State of Thuringia, Germany, and by the Collaborative Research Centre 1076 “AquaDiva” from the Deutsche Forschungsgemeinschaft (DFG) is highly acknowledged. B.M. and M-C.G. thank the student workers for their assistance during the experimental work.


  1. 1.
    Michalzik B (2011) Insects Infestations Nutr Fluxes 216:557–580Google Scholar
  2. 2.
    Dale VH, Joyce LA, McNulty S, Neilson RP, Ayres MP, Flannigan MD, Hanson PJ, Irland LC, Lugo AE, Peterson CJ, Simberloff D, Swanson FJ, Stocks BJ, Michael Wotton B (2001) Climate change and forest disturbances. Bio Sci 51(9):723Google Scholar
  3. 3.
    Seidl R, Rammer W, Jäger D, Lexer MJ (2008) Impact of bark beetle (Ips typographus L.) disturbance on timber production and carbon sequestration in different management strategies under climate change. For Ecol Manag 256(3):209–220CrossRefGoogle Scholar
  4. 4.
    Meinke I (2010) Klimawandel an der deutschen Ostseeküste. Meer und Küste, p 2Google Scholar
  5. 5.
    McDowell N, Pockman WT, Allen CD, Breshears DD, Cobb N, Kolb T, Plaut J, Sperry J, West A, Williams DG, Yepez EA (2008) Mechanisms of plant survival and mortality during drought: why do some plants survive while others succumb to drought? New Phytol 178(4):719–739CrossRefGoogle Scholar
  6. 6.
    Bréda N, Cochard H, Dreyer E, Granier A (1993) Water transfer in a mature oak stand (Quercuspetraea): seasonal evolution and effects of a severe drought. Can J For Res 23(6):1136–1143CrossRefGoogle Scholar
  7. 7.
    Pretzsch, H.; Schutze, G; Uhl, E (2012) Resistance of European tree species to drought stress in mixed versus pure forests: evidence of stress release by inter-specific facilitation. Plant Biol (Stuttg) Google Scholar
  8. 8.
    Frosch T, Koncarevic S, Becker K, Popp J (2009) Morphology-sensitive Raman modes of the malaria pigment hemozoin. Analyst 134(6):1126–1132CrossRefGoogle Scholar
  9. 9.
    Frosch T, Popp J (2009) Relationship between molecular structure and Raman spectra of quinolines. J Mol Struct 924–926:301–308CrossRefGoogle Scholar
  10. 10.
    Frosch T, Popp J (2010) Structural analysis of the antimalarial drug halofantrine by means of Raman spectroscopy and density functional theory calculations. J Biomed Opt 15(4):041516–041516CrossRefGoogle Scholar
  11. 11.
    Frosch T, Yan D, Popp J (2013) Ultrasensitive fiber enhanced UV resonance Raman sensing of drugs. Anal Chem 85(13):6264–6271CrossRefGoogle Scholar
  12. 12.
    Frosch T, Keiner R, Michalzik B, Fischer B, Popp J (2013) Investigation of gas exchange processes in peat bog ecosystems by means of innovative Raman gas spectroscopy. Anal Chem 85(3):1295–1299CrossRefGoogle Scholar
  13. 13.
    Keiner R, Frosch T, Massad T, Trumbore S, Popp J (2014) Enhanced Raman multigas sensing—a novel tool for control and analysis of (13)CO2 labeling experiments in environmental research. Analyst (Cambridge, U K) 139(16):3879–3884CrossRefGoogle Scholar
  14. 14.
    Hanf S, Keiner R, Yan D, Popp J, Frosch T (2014) Fiber-enhanced Raman multigas spectroscopy: a versatile tool for environmental gas sensing and breath analysis. Anal Chem 86(11):5278–5285CrossRefGoogle Scholar
  15. 15.
    King DA, Pittaro RJ (1998) Simple diode pumping of a power-buildup cavity. Opt Lett 23(10):774–776CrossRefGoogle Scholar
  16. 16.
    Keiner R, Frosch T, Hanf S, Rusznyak A, Akob DM, Kusel K, Popp J (2013) Raman spectroscopy—an innovative and versatile tool to follow the respirational activity and carbonate biomineralization of important cave bacteria. Anal Chem 85:8708–8714CrossRefGoogle Scholar
  17. 17.
    Hanf R, Bögözi T, Keiner R, Frosch T, Popp J (2015) Fast and highly sensitive fiber enhanced Raman spectroscopic monitoring of molecular H2 and CH4 for point-of-care diagnosis of malabsorption disorders in exhaled human breath. Anal Chem. doi: 10.1021/ac503450y
  18. 18.
    Peuke AD, Gessler A, Rennenberg H (2006) The effect of drought on C and N stable isotopes in different fractions of leaves, stems and roots of sensitive and tolerant beech ecotypes. Plant, Cell Environ 29(5):823–835CrossRefGoogle Scholar
  19. 19.
    Edwards JS (1966) Defence by smear: supercooling in the cornicle wax of aphids. Nature 211(5044):73–74CrossRefGoogle Scholar
  20. 20.
    Smith RG (1999) Wax glands, wax production and the functional significance of wax use in three aphid species (Homoptera: Aphididae). J Nat Hist 33(4):513–530CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Robert Keiner
    • 1
    • 2
  • Marie-Cécile Gruselle
    • 3
    • 4
  • Beate Michalzik
    • 3
  • Jürgen Popp
    • 1
    • 2
    • 5
  • Torsten Frosch
    • 1
    • 2
    Email author
  1. 1.Leibniz Institute of Photonic TechnologyJenaGermany
  2. 2.Institute for Physical ChemistryFriedrich-Schiller UniversityJenaGermany
  3. 3.Institute of GeographyFriedrich-Schiller UniversityJenaGermany
  4. 4.School of Forest ResourcesUniversity of MaineOronoUSA
  5. 5.Abbe Centre of PhotonicsFriedrich Schiller UniversityJenaGermany

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