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Analytical and Bioanalytical Chemistry

, Volume 407, Issue 7, pp 1901–1912 | Cite as

Cross-platform metabolic profiling: application to the aquatic model organism Lymnaea stagnalis

  • Sara TufiEmail author
  • Marja H. Lamoree
  • Jacob De Boer
  • Pim E. G. Leonards
Research Paper

Abstract

The freshwater pond snail Lymnaea stagnalis is used in several studies on molecular and behavioral neurobiology and ecotoxicology showing its successful application as a model organism. In the present study, a cross-platform metabolomic approach has been evaluated to characterize the organ molecular phenotypes of L. stagnalis central nervous system (CNS), digestive gland (DG), and albumen gland (AG). Two types of tissue disruption methods were evaluated of which beads beating was the preferred method. To obtain a broad picture of the hydrophilic and lipophilic metabolome, two complementary analytical platforms have been employed: liquid chromatography (LC) and gas chromatography (GC) coupled to high-resolution mass spectrometry. Furthermore, to increase the power to separate small polar metabolites, hydrophilic interaction liquid chromatography (HILIC) was applied. The analytical platform performances have been evaluated based on the metabolome coverage, number of molecular features, reproducibility, and multivariate data analysis (MVDA) clustering. This multiplatform approach is a starting point for future global metabolic profiling applications on L. stagnalis.

Keywords

Metabolomics Lymnaea stagnalis Cross-platform LC-MS GC-MS HILIC 

Notes

Acknowledgments

This study was carried out within the Marie Curie Research Training Network EDA-EMERGE (www.eda-emerge.eu) supported by the EU (MRTN-CT-2012-290100).

Supplementary material

216_2014_8431_MOESM1_ESM.pdf (570 kb)
ESM 1 (PDF 570 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sara Tufi
    • 1
    Email author
  • Marja H. Lamoree
    • 1
  • Jacob De Boer
    • 1
  • Pim E. G. Leonards
    • 1
  1. 1.Institute for Environmental Studies (IVM)VU University AmsterdamAmsterdamThe Netherlands

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