Analytical and Bioanalytical Chemistry

, Volume 407, Issue 11, pp 2965–2974 | Cite as

Liquid chromatography with isotope-dilution mass spectrometry for determination of water-soluble vitamins in foods

  • Melissa M. PhillipsEmail author
Paper in Forefront
Part of the following topical collections:
  1. Reference Materials for Chemical Analysis


Vitamins are essential for improving and maintaining human health, and the main source of vitamins is the diet. Measurement of the quantities of water-soluble vitamins in common food materials is important to understand the impact of vitamin intake on human health, and also to provide necessary information for regulators to determine adequate intakes. Liquid chromatography (LC) and mass spectrometry (MS) based methods for water-soluble vitamin analysis are abundant in the literature, but most focus on only fortified foods or dietary supplements or allow determination of only a single vitamin. In this work, a method based on LC/MS and LC/MS/MS has been developed to allow simultaneous quantitation of eight water-soluble vitamins, including multiple forms of vitamins B3 and B6, in a variety of fortified and unfortified food-matrix Standard Reference Materials (SRMs). Optimization of extraction of unbound vitamin forms and confirmation using data from external laboratories ensured accuracy in the assigned values, and addition of stable isotope labeled internal standards for each of the vitamins allowed for increased precision.


Vitamins Nutrition Fortification Food Mass spectrometry Isotope dilution 



The author would like to thank the members and staff of the Grocery Manufacturer’s Association Food Industry Analytical Chemists (FIAC) Share Group for their contribution of data used in the assignment of the values for water-soluble vitamins in these various food-matrix SRMs. The author would also like to thank Katherine Sharpless (NIST) and James Yen (NIST) for compilation and statistical analysis, respectively, of the data used in the determination of the final certified and reference values. The author also acknowledges Barbara Porter (NIST), Michele Schantz (NIST), and Brian Lang (NIST) for their contributions to determination of the purity of the water-soluble vitamin reference standards used in these studies. The author also greatly appreciates helpful discussions with Catherine Rimmer (NIST) and Stephen Wise (NIST), and the overall coordination of the numerous SRM projects by Lane Sander (NIST), Katherine Sharpless (NIST), Stephen Wise (NIST), and Laura Wood (NIST).

Conflict of interest

The author declares no competing financial interest.

Supplementary material

216_2014_8354_MOESM1_ESM.pdf (39 kb)
ESM 1 (PDF 39 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2014

Authors and Affiliations

  1. 1.Chemical Sciences Division, Material Measurement LaboratoryNational Institute of Standards and TechnologyGaithersburgUSA

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