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Biotin–streptavidin-amplified real-time immune-PCR assay for detecting dimethyl phthalate in beverage and drinking water samples

Analytical and Bioanalytical Chemistry volume 407, pages 1261–1265 (2015)Cite this article

Abstract

Dimethyl phthalate (DMP), one family of the phthalic acid diesters (PAEs), is an increasing widely used plasticizer. A sensitive and high-throughput direct competitive biotin–streptavidin-amplified system based on real-time immune-PCR (BA-rt-IPCR) techniques was developed for detecting DMP in beverage and drinking water samples. In our assay, we selected dimethyl 4-aminophthalate as the optimal DMP hapten to prepare high titer of rabbit polyclonal anti-DMP antibodies (pAb-DMP). Under the optimized conditions, the proposed method was used to detect DMP with a linearity range from 10 to 100 ng L−1, and the limit of detection (LOD) was 1.98 pg L−1. Finally, the results about DMP in beverage and drinking water samples were consistent with those using gas chromatography–mass spectrometry (GC-MS), which proved that the proposed immunoassay for detecting DMP in the environment was accurate, reliably rapid, and receptive.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Project No. 21177082), the Science and Technology Commission of Shanghai Municipality in China (Key Project of Fundamental Research No. 09JC1407600), and Shanghai Jiao Tong University Science and Technology Innovation special fund development projects.

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Affiliations

  1. School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China

    Ruiyan Sun & Huisheng Zhuang

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  1. Ruiyan Sun
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  2. Huisheng Zhuang
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Correspondence to Huisheng Zhuang.

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Sun, R., Zhuang, H. Biotin–streptavidin-amplified real-time immune-PCR assay for detecting dimethyl phthalate in beverage and drinking water samples. Anal Bioanal Chem 407, 1261–1265 (2015). https://doi.org/10.1007/s00216-014-8329-z

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  • DOI: https://doi.org/10.1007/s00216-014-8329-z

Keywords

  • Dimethyl phthalate (DMP)
  • Biotin–streptavidin-amplified system
  • Real-time immuno-PCR
  • Beverage and drinking water samples