Analytical and Bioanalytical Chemistry

, Volume 407, Issue 11, pp 2997–3007 | Cite as

T-2 and HT-2 toxins in oat flakes: development of a certified reference material

  • Robert KöppenEmail author
  • Wolfram Bremser
  • Ina Stephan
  • Karin Klein-Hartwig
  • Tatjana Rasenko
  • Matthias Koch
Research Paper
Part of the following topical collections:
  1. Reference Materials for Chemical Analysis


Trichothecene mycotoxins, with T-2 and HT-2 toxins being the main representatives of the type A subgroup, are naturally and worldwide occurring contaminants frequently found in grain-based food and feed. Due to the high consumption of these products and the potential health risk associated herewith, concerns about the safety and quality of food and feed have increased over the last decades at both governmental and consumer levels. Since it is not possible to avoid their occurrence, tremendous efforts have been performed to identify and monitor mycotoxins in food and feed to make their consumption safe. However, suitable certified reference materials (CRMs) intended for quality assurance and quality control purposes are still lacking for many mycotoxin-matrix combinations. Therefore, in the framework of a European Reference Material (ERM®) project, the first CRM for T-2 and HT-2 toxin in ground oat flakes (ERM®-BC720) was developed according to the requirements of ISO Guide 35. The whole process of ERM®-BC720 development, including sample preparation, homogeneity and stability studies and value assignment, is presented. The assignment of the certified mass fractions was based upon an in-house study using high-performance liquid chromatography isotope-dilution tandem mass spectrometry. Simultaneously, an interlaboratory comparison study involving 24 expert laboratories was conducted in order to support the in-house certification study. The certified values and their corresponding expanded uncertainties (k = 2) for both T-2 and HT-2 toxin in ERM®-BC720, traceable to the international system of units, are (82 ± 4) μg kg−1 and (81 ± 4) μg kg−1, respectively.


Type A trichothecenes Fusarium toxins Isotope-dilution mass spectrometry Food analysis European Reference Material Quality assurance 



We gratefully thank the mentioned laboratories for participation in the ILC.

Conflicts of interest

There are no conflicts of interest.

Supplementary material

216_2014_8251_MOESM1_ESM.pdf (28 kb)
ESM 1 (PDF 28.4 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Robert Köppen
    • 1
    Email author
  • Wolfram Bremser
    • 1
  • Ina Stephan
    • 1
  • Karin Klein-Hartwig
    • 1
  • Tatjana Rasenko
    • 1
  • Matthias Koch
    • 1
  1. 1.Division 1.7-Food AnalysisFederal Institute for Materials Research and Testing (BAM)BerlinGermany

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