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Analytical and Bioanalytical Chemistry

, Volume 406, Issue 25, pp 6347–6362 | Cite as

Applicability of UV laser-induced solid-state fluorescence spectroscopy for characterization of solid dosage forms

  • Eva Woltmann
  • Hans Meyer
  • Diana Weigel
  • Heinz Pritzke
  • Tjorben N. Posch
  • Pablo A. Kler
  • Klaus Schürmann
  • Jörg Roscher
  • Carolin HuhnEmail author
Research Paper

Abstract

High production output of solid pharmaceutical formulations requires fast methods to ensure their quality. Likewise, fast analytical procedures are required in forensic sciences, for example at customs, to substantiate an initial suspicion. We here present the design and the optimization of an instrumental setup for rapid and non-invasive characterization of tablets by laser-induced fluorescence spectroscopy (with a UV-laser (λ ex = 266 nm) as excitation source) in reflection geometry. The setup was first validated with regard to repeatability, bleaching phenomena, and sensitivity. The effect on the spectra by the physical and chemical properties of the samples, e.g. their hardness, homogeneity, chemical composition, and granule grain size of the uncompressed material, using a series of tablets, manufactured in accordance with design of experiments, was investigated. Investigation of tablets with regard to homogeneity, especially, is extremely important in pharmaceutical production processes. We demonstrate that multiplicative scatter correction is an appropriate tool for data preprocessing of fluorescence spectra. Tablets with different physical and chemical characteristics can be discriminated well from their fluorescence spectra by subjecting the results to principal component analysis.

Keywords

Tablet analysis Solid-state fluorescence spectroscopy Grain size Photobleaching Scattering effects Homogeneity 

Notes

Acknowledgements

We thank Jennifer Oppenberg (University of Münster, Germany) for her introduction to the fabrication of tablets. The help of Dr Jan Pöggeler (Forschungszentrum Jülich, Germany) is gratefully acknowledged. This project was partially funded by the Federal Ministry of Education and Research (BMBF), FZK: 13 N12012. This work was partially funded by the Excellence Initiative, a jointly funded program of the German federal and state governments, organized by the German Research Foundation (DFG).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Eva Woltmann
    • 1
  • Hans Meyer
    • 2
  • Diana Weigel
    • 3
  • Heinz Pritzke
    • 4
  • Tjorben N. Posch
    • 1
  • Pablo A. Kler
    • 1
    • 5
  • Klaus Schürmann
    • 6
  • Jörg Roscher
    • 7
  • Carolin Huhn
    • 1
    • 5
    Email author
  1. 1.Central Institute for Engineering, Electronics and Analytics: Analytics (ZEA-3)Forschungszentrum JülichJülichGermany
  2. 2.J&M Analytik AGEssingenGermany
  3. 3.Federal Criminal Police OfficeForensic Science Institute, KT 34 – ToxicologyWiesbadenGermany
  4. 4.Glatt Systemtechnik GmbHDresdenGermany
  5. 5.Institute of Physical and Theoretical ChemistryEberhard Karls Universität TübingenTübingenGermany
  6. 6.LabCognition Analytical Software GmbH & Co. KGKölnGermany
  7. 7.Institute of Inorganic and Analytical ChemistryWestfälische Wilhelms-Universität MünsterMünsterGermany

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